Temperature and Glycerol Formation: A Proposal to Explain the Causal Relationship Based on Glycolytic Enzyme Activities

Supplemental Table 1   Values of kinetic parameters used in the model.

Supplemental Table 2  Values of parameters of PFK kinetics (Teusink et al. 2000).

Supplemental Table 3  Specific activities determined in vitro at 30°C. Vmax values of HXT and GLYCPASE are taken from literature.

Supplemental Table 4   Values of parameters fitted for the effect of ethanol on enzyme activities.

Supplemental Figure 1  Arrhenius plots of the enzymes of the glycolysis pathway and its glycerol and ethanol branches. Plots were drawn using the specific activity data determined in vitro at 10, 15, 20, 25, 30, and 35°C. ADH: alcohol dehydrogenase, ALD: aldolase, ENO: enolase, GAPDH: glyceraldehyde-3-phosphate dehydrogenase, GLYC3PDH: glycerol-3-phosphate dehydrogenase, HXK: hexokinase, PDC: pyruvate decarboxylase, PFK: phosphofructokinase, PGI: phosphoglucose isomerase, PGK: phosphoglycerate kinase, PGM: phosphoglycerate mutase, PYK: pyruvate kinase, TPI: triose phosphate isomerase.

Supplemental Figure 2  Effect of ethanol on enzyme activities. ADH: alcohol dehydrogenase, ALD: aldolase, ENO: enolase, GAPDH: glyceraldehyde-3-phosphate dehydrogenase, GLYC3PDH: glycerol-3-phosphate dehydrogenase, HXK: hexokinase, PDC: pyruvate decarboxylase, PFK: phosphofructokinase, PGI: phosphoglucose isomerase, PGK: phosphoglycerate kinase, PGM: phosphoglycerate mutase, PYK: pyruvate kinase, TPI: triose phosphate isomerase.

Appendix:  Enzyme Assays

Files in this Data Supplement:

• Supplemental_Data.pdf