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Grapevine Non-vinifera Genetic Diversity Assessed by Simple Sequence Repeat Markers as a Starting Point for New Rootstock Breeding Programs

Daniele Migliaro, Gabriella De Lorenzis, Giovambattista Simone Di Lorenzo, Barbara De Nardi, Massimo Gardiman, Osvaldo Failla, Lucio Brancadoro, Manna Crespan
Am J Enol Vitic. October 2019 70: 390-397; published ahead of print June 07, 2019 ; DOI: 10.5344/ajev.2019.18054
Daniele Migliaro
1CREA – Research Centre for Viticulture and Enology, Viale XXVIII Aprile, 26 – 31015 Conegliano (Treviso), Italy
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Gabriella De Lorenzis
2Department of Agricultural and Environmental Sciences, Via Celoria, 2 – 20133 Milano, Italy.
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Giovambattista Simone Di Lorenzo
2Department of Agricultural and Environmental Sciences, Via Celoria, 2 – 20133 Milano, Italy.
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Barbara De Nardi
1CREA – Research Centre for Viticulture and Enology, Viale XXVIII Aprile, 26 – 31015 Conegliano (Treviso), Italy
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Massimo Gardiman
1CREA – Research Centre for Viticulture and Enology, Viale XXVIII Aprile, 26 – 31015 Conegliano (Treviso), Italy
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Osvaldo Failla
2Department of Agricultural and Environmental Sciences, Via Celoria, 2 – 20133 Milano, Italy.
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Lucio Brancadoro
2Department of Agricultural and Environmental Sciences, Via Celoria, 2 – 20133 Milano, Italy.
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Manna Crespan
1CREA – Research Centre for Viticulture and Enology, Viale XXVIII Aprile, 26 – 31015 Conegliano (Treviso), Italy
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  • For correspondence: manna.crespan@crea.gov.it
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Data supplements

  • Supplemental Text
    Supplemental Text 1:  Selection of the most suitable set of simple sequence repeat (SSR) loci for the genotyping of rootstocks.
    Supplemental Text 2:  Detailed description of genotype identification.
    Supplemental Text 3:  Detailed description of kinship results.

    Supplemental Figure 1   Genotype accumulation curve of 232 grapevine rootstock accessions, genotyped over 16 SSR loci.

    Supplemental Table 1   List of 379 rootstock accessions genotyped with 17 simple sequence repeat (SSR) loci. The accessions are grouped based on their genotype. Ascertained misnomered accessions are written in italic. Flower sex and donor institutes of the accessions are reported, as well as variety name as resulted from genotyping, pedigree information from the literature, and present molecular data.

    Supplemental Table 2   Three multiplex panels for PCR.

    Supplemental Table 3  Allelic profiles at 17 simple sequence repeat (SSR) loci of 232 grapevine rootstock genotypes. Original questionable accession names are converted to “no name.” Suggested names for uncertain varieties are in bold.

    Supplemental Table 4  List of alleles found at each of the 17 simple sequence repeat loci analyzed.

    Supplemental Table 5  Genotypes subdivision depending on cluster membership (Q > 85%) and admixed (Q < 0.85) according to structure. Genotypes are in alphabetical order.

    Supplemental Table 6  List of the genotypes included in nine different core collections obtained from a dataset of 232 grapevine rootstock genotypes. Simple sequence repeat (SSR) profile ID according to Supplemental Table 5.

    • Supplemental_Text.pdf
    • Supplemental_Figure_1.pdf
    • Supplemental_Table_1.pdf
    • Supplemental_Table_2.pdf
    • Supplemental_Table_3.pdf
    • Supplemental_Table_4.pdf
    • Supplemental_Table_5.pdf
    • Supplemental_Table_6.pdf
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Vol 70 Issue 4

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Grapevine Non-vinifera Genetic Diversity Assessed by Simple Sequence Repeat Markers as a Starting Point for New Rootstock Breeding Programs
Daniele Migliaro, Gabriella De Lorenzis, Giovambattista Simone Di Lorenzo, Barbara De Nardi, Massimo Gardiman, Osvaldo Failla, Lucio Brancadoro, Manna Crespan
Am J Enol Vitic.  October 2019  70: 390-397;  published ahead of print June 07, 2019 ; DOI: 10.5344/ajev.2019.18054

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Grapevine Non-vinifera Genetic Diversity Assessed by Simple Sequence Repeat Markers as a Starting Point for New Rootstock Breeding Programs
Daniele Migliaro, Gabriella De Lorenzis, Giovambattista Simone Di Lorenzo, Barbara De Nardi, Massimo Gardiman, Osvaldo Failla, Lucio Brancadoro, Manna Crespan
Am J Enol Vitic.  October 2019  70: 390-397;  published ahead of print June 07, 2019 ; DOI: 10.5344/ajev.2019.18054
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