Skip to main content
Advertisement

Main menu

  • Home
  • Content
    • Current Volume
    • AJEV and Catalyst Archive
    • Best Papers
    • ASEV National Conference Technical Abstracts
    • Collections
    • Free Sample Issue
    • Print on Demand
  • Information For
    • Authors
    • Open Access and Subscription Publishing
    • Submission
    • Subscribers
      • Proprietary Rights Notice for AJEV Online
    • Permissions and Reproductions
  • About Us
  • Feedback
  • Alerts
  • Help
  • Login
  • ASEV MEMBER LOGIN

User menu

  • Log in

Search

  • Advanced search
American Journal of Enology and Viticulture
  • Log in
  • Follow ajev on Twitter
  • Follow ajev on Linkedin
American Journal of Enology and Viticulture

Advanced Search

  • Home
  • Content
    • Current Volume
    • AJEV and Catalyst Archive
    • Best Papers
    • ASEV National Conference Technical Abstracts
    • Collections
    • Free Sample Issue
    • Print on Demand
  • Information For
    • Authors
    • Open Access and Subscription Publishing
    • Submission
    • Subscribers
    • Permissions and Reproductions
  • About Us
  • Feedback
  • Alerts
  • Help
  • Login
  • ASEV MEMBER LOGIN
Research Article

Effects of Frozen Materials Other Than Grapes on Red Wine Aroma Compounds. Impacts of Harvest Technologies

Yibin Lan, Jiaming Wang, Emily Aubie, Marnie Crombleholme, Andrew Reynolds
Am J Enol Vitic.  2022 73: 142-155 ; DOI: 10.5344/ajev.2022.22006
Yibin Lan
1Center for Viticulture and Enology, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China;
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Jiaming Wang
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
3Postdoctoral Fellow. Present address: Labstat International Inc., Kitchener, ON;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Emily Aubie
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
4Postdoctoral Fellow. Present address: Andrew Peller Winery, 697 S Service Rd, Grimsby, ON L3M 4E8, Canada;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Marnie Crombleholme
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
5Undergraduate thesis student. Present address: Quai du Vin Winery, St. Thomas, ON; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Andrew Reynolds
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
6Former Professor. Present address: 613 Memorial Drive, Fenwick, ON L0S1C0; All research work was completed at Cool Climate Oenology & Viticulture Institute, Brock University.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
  • For correspondence: andrewreynolds2007@gmail.com
  • Article
  • Figures & Data
  • Supplemental
  • Info & Metrics
  • PDF
Loading

Abstract

An undesirable sensory attribute (“floral taint”) has been detected in red wines in North America, caused by leaves and petioles (materials other than grapes [MOG]) introduced during mechanical harvest after killing frosts. From 2017 to 2019, several harvest strategies were evaluated on Ontario Cabernet franc: hand harvest (HH), conventional machine harvesting (MECH), Braud-New Holland Opti (OPTI), Gregoire GL8, MECH + optical sorting (MECH+OS), and MECH with preharvest leaf removal (MECH+BLR). Concentrations of 41 odor-active compounds were quantified by gas chromatography-mass spectrometry. Harvest treatment effects varied by season. In 2017, HH resulted in lowest ethyl isobutyrate (MECH+BLR), ethyl nonoate, cis-linalool oxide (plus MECH and OPTI), trans-linalool oxide (plus MECH+OS), β-citral, and cis- and trans-rose oxide (plus MECH and OPTI). Ethyl hexanoate was lowest in MECH, and MECH+BLR, isoamyl hexanoate was lowest in all treatments except HH, and α-ionone was lowest in MECH and MECH+BLR. In 2018, HH resulted in the lowest β-damascenone, ethyl salicylate (plus OPTI and Gregoire), citronellol (plus Gregoire), cis- and trans-rose oxide (plus Gregoire), and eugenol (plus Gregoire). Isobutyl acetate, isoamyl hexanoate, and nerol were additionally reduced by Gregoire, and isopropylmethoxypyrazine was reduced by all treatments except HH. In 2019, harvest strategy affected 27 of 41 compounds, including 11 esters and 12 terpenes. Treatments leading to lowest concentrations were HH (nine compounds), MECH (eight compounds), MECH+BLR (10 compounds), OPTI (21 compounds), Gregoire (10 compounds), and MECH+OS (22 compounds). Wines from fruit that had undergone a killing frost contained different concentrations of 14 and eight compounds (2018), and 17 and 13 compounds (2019) for Cabernet franc and Cabernet Sauvignon, respectively. Results suggest that specific harvest technologies can reduce MOG and associated increases in aroma compounds, although seasonal differences may occur.

  • esters
  • mechanical harvest
  • methoxypyrazines
  • norisoprenoids
  • onoterpenes
  • optical sorting

Mechanized harvesting has been widely used in many grapegrowing regions for the past 50 years, but has frequently resulted in greater amounts of materials other than grapes (MOG) in harvest loads. Presence of MOG, e.g., leaves and petioles, can substantially diminish the quality and composition of wines, particularly red wines (Noble et al. 1975, Huang et al. 1988, Guerrini et al. 2018). Many studies were conducted worldwide with the advent of mechanical harvesting in the 1970s (e.g., Christensen et al. 1973, Petrucci and Siegfried 1976, Johnson 1977, Peterson 1979). Normally, mechanized harvest was considered equal to hand harvesting with respect to wine quality; however, most early mechanical harvesters were unable to expel all the MOG accumulated during harvest. Current harvesting technology has largely eliminated this problem except under unusual circumstances, such as with frost-damaged canopies (Parenti et al. 2015, Hendrickson et al. 2016). Many new mechanical harvesting technologies have optical sorting capabilities that will eject leaves, petioles, and unripe fruit (Hendrickson and Oberholster 2017, Kilmartin and Oberholster 2022). However, this equipment may not be the best financial option for grapegrowers to avoid sensory taints. Modifying vineyard management strategies such as time or method of harvest, or hand-picking specific grape varieties, may be suitable alternatives that can mitigate adverse effects of late-season mechanized harvest. This may encourage equipment companies to tailor products more toward individual grape varieties and the level of product quality, as well as the purchasing ability, anticipated by wineries. In some situations, a grapegrower with a modest acreage may choose custom harvesting using new equipment with sorting capabilities rather than purchasing a new harvester.

Some grape cultivars (e.g., Cabernet franc) are especially prone to a unique sensory defect, locally referred to as “floral taint,” introduced by MOG following killing frosts. Warmer autumns, perhaps due to climate change, have permitted harvests of red wine cultivars such as Cabernet franc as late as mid- to late- November, when foliage had heretofore been killed by frost. It is highly likely that undesirable aroma compounds, e.g., monoterpenes and norisoprenoids, can be introduced by frozen leaves and petioles, and postfrost machine harvesting is a major contributor (Wang et al. 2020). These sensory taints, along with enabling grapegrowers to adapt to a changing climate, must both be addressed; therefore, the wine industry must understand the effects of frozen MOG on basic wine composition and wine aroma compounds, and identify harvest technologies that will minimize its incorporation into harvest loads.

Mechanical harvesting can adversely affect physical characteristics of fruit including berry damage, premature juice release, and increased MOG content (Pezzi et al. 2008, Kilmartin and Oberholster 2022). Differences in one study were largely attributed to oxidation during prolonged transport, more than differences between hand-harvested versus machine-harvested wines (Pezzi et al. 2008). Mechanical harvest results in the release of specific enzymes from both fruit and MOG, and these enzymes may come into contact with substrates (Kilmartin and Oberholster 2022). Conventional must and wine composition can consequently be adversely affected by MOG incorporation including ethanol, titratable acidity (TA), pH, malic acid, total phenol concentration, and color (Arfelli et al. 2010, Ward et al. 2015, Guerrini et al. 2018). Wine ethanol concentration can be substantially reduced with high MOG levels (Ward et al. 2015, Guerrini et al. 2018), and the presence of MOG during fermentation can additionally result in wines with higher pH and lower TA, albeit with higher malic acid concentrations (Arfelli et al. 2010, Ward et al. 2015, Guerrini et al. 2018). Incorporation of MOG into the must also has an adverse effect on color and phenolic concentration of red winegrape varieties (Wagener 1980, Huang et al. 1988, Guerrini et al. 2018). Phenolic compounds, e.g., flavanoids and tannins, can transfer from MOG into fermenting wines (Spranger et al. 2004, Suriano et al. 2016). MOG, especially petioles that are incorporated into fermentations, can also absorb anthocyanins from the fermenting must, resulting in decreased color intensity (Pascual et al. 2016, Suriano et al. 2016). However, increased MOG is frequently associated with an increase in total phenols, mainly due to elevated flavonoid concentrations in MOG-affected wines (Huang et al. 1988, Arfelli et al. 2010, Guerrini et al. 2018). High MOG levels can consequently also lead to increases in the tannin and anthocyanin concentrations in wines, and as a result, inclusion of MOG has an adverse effect on the hue and color intensity (Wagener 1980, Huang et al. 1988, Guerrini et al. 2018), often in fact leading to increases in both color intensity and hue (Guerrini et al. 2018). These alterations in the chemical composition can potentially influence the overall wine sensory profile and quality (Noble et al. 1975, Guerrini et al. 2018).

MOG may likewise affect concentrations of wine aroma compounds (Kilmartin and Oberholster 2022). The concentration and composition of volatile compounds vary depending on grapevine organ, especially among the vegetative tissues (e.g., stems, tendrils, rachis, peduncles), in addition to the berries themselves (Matarese et al. 2014). The volatile compound composition associated with the rachis, peduncles, stems, and tendrils are composed mainly of monoterpenes such as geraniol, linalool, and β-citronellol (Matarese et al. 2014). Remaining organs including leaves are composed mostly of aliphatic C6 compounds such as hexanal; 2-hexenal; 2,4-heptadienal; 2,4-hexadienal; plus 1-octen-3-ol; which are characterized by a green odor (Matarese et al. 2014). Other volatile compounds contributed by MOG are present at lower concentrations, e.g., methyl salicylate, benzyl alcohol, benzaldehyde, 2-phenylethanol, various norisoprenoids, and eugenol (Gunata et al. 1986, Matarese et al. 2014). Harvested fruit can likewise contribute to high concentrations of C6 compounds as a result of lipoxygenase activity (Kilmartin and Oberholster 2022).

Early studies found few sensory or aroma compound differences among hand-harvested and mechanically-harvested treatments (Noble et al. 1975). No increases were reported in the concentrations of leaf volatiles, such as trans-3-hexenal and cis-3-hexenol in machine-harvested must, and no sensory differences were found between hand-harvested and machine-harvested wines (Joslin and Ough 1978). However, more recent work with Pinot noir using optical berry sorting in combination with mechanized harvest demonstrated positive effects of the mechanized harvest method as well as postharvest optical sorting (Hendrickson et al. 2016, Hendrickson and Oberholster 2017). Hand-harvested fruit that was subsequently sorted produced wines with the highest dark-fruit aroma, while mechanical harvest that included the Selectiv’ process (onboard MOG removal) led to higher tropical fruit aromas (Hendrickson et al. 2016, Kilmartin and Oberholster 2022). Mechanically-harvested grapes had higher concentrations of linalool, β-myrcene, α-terpinene, and β-damascenone, presumably due to glycosidic hydrolysis triggered by berry damage during harvest (Hendrickson et al. 2016). Differences in wine composition attributable to harvest treatment were reduced by postharvest optical sorting.

Floral sensory taints, observed in Ontario’s commercial red wines, have been associated with mechanically-harvested grapes, so therefore it was hypothesized that increased concentrations of undesirable aroma compounds such as monoterpenes and norisoprenoids are being introduced by frozen leaves and petioles. Despite being present in very small concentrations, terpenes can have a considerable effect on the sensory properties of grapes and wines. Floral monoterpene-based aromas are typical and desirable in white wines such as Muscat, Riesling, and Gewürztraminer (Marais 1983), however, they are atypical in red table wines (Ferreira et al. 2000). It was estimated that >5% of petioles were required to substantially alter sensory attributes, particularly increasing terpene-based floral aromas in Cabernet Sauvignon (Ward et al. 2015). It is possible that prolonged autumn seasons associated with climate change, while permitting harvests later in the season, also allow for odor-active compounds such as terpenes to develop to undesirable concentrations in mature fruit. These compounds may likewise be introduced through processes such as postfrost machine harvesting. It was also hypothesized that methoxypyrazines would be reduced because of light freezing of grapes that could occur along with late harvesting, consistent with local industry anecdotal evidence. The objectives of this study were to investigate the effect of several harvesting strategies on Cabernet franc for their ability to reduce frozen MOG that negatively affects wine composition and quality. The second objective was to specifically assess the effect of freezing itself on the concentrations of aroma compounds in Cabernet franc and Cabernet Sauvignon.

A preliminary analysis of the effect of frozen MOG has appeared (Wang et al. 2020). This work extends that study to three vintages, 2017, 2018, and 2019, with a more robust data analysis.

Materials and Methods

Harvesting treatments. All Cabernet franc (Vitis vinifera L.) grapes were harvested following a hard frost in the 2017 to 2019 seasons from the Andrew Peller Ltd. Carlton St. vineyards, located in the Niagara Peninsula Vintners Quality Alliance (VQA) subappellation of Four Mile Creek in Niagara-On-The-Lake, Ontario, Canada. Entire rows (≈750 kg of fruit) were designated for each treatment. Treatments were hand harvest (HH), conventional mechanical harvest (MECH), MECH preceded by full canopy mechanical leaf removal (MECH+BLR), Braud-New Holland 9060L MECH with Opti-Grape system (OPTI; CHN Industrial), Gregoire GL8 MECH with EasyClean Destemmer Sorter (2018, 2019 only; SDF Group), and MECH followed by in-winery optical sorting (MECH+OS; 2017, 2019 only; Selectiv’ Process Vision 2, Pellenc ST). Conventional MECH and MECH+BLR were performed in 2017 with a Braud-New Holland BRAUD 9060L without the Opti-Grape system implemented; in 2018 and 2019, MECH was performed with a Gregoire GL8 without the EasyClean Destemmer Sorter option implemented. Following harvest and/or in-winery optical sorting, grapes from each treatment were divided equally into three replicates for subsequent winemaking. Harvest dates were 14 Nov 2017, 11 Nov 2018, and 13 Nov 2019.

Frost treatments. Cabernet franc and Cabernet Sauvignon grapes were harvested prior to and following a hard frost in the 2018 to 2019 seasons from the Andrew Peller Carlton St. vineyards. Entire rows (≈750 kg of fruit) were designated for each treatment. Following harvest, grapes from each treatment were divided equally into three replicates for subsequent winemaking. Pre- and postfrost harvest dates were 4 versus 13 Nov 2018, and 5 versus 16 Nov 2019 for both cultivars).

Winemaking. Following destemming, must was treated with 50 mg/L potassium metabisulfite. Fermentations were performed in triplicates of 40 kg in 46-L plastic fermentation buckets. The fermentation vessels were then placed in a 24°C fermentation chamber and allowed to warm up overnight. Juice samples were taken immediately prior to inoculation and frozen at -25°C for future analysis. Fermentations were inoculated with 350 mg/L of yeast strain CSM (Lallemand) one day after harvest. An addition of 200 mg/L diammonium phosphate was made 24 hrs after inoculation. Fermentations were hand plunged twice daily, and fermentation kinetics (sugar concentration and temperature) were monitored daily. Seven days after destemming, the must was pressed and the wine was inoculated with the malolactic bacteria strain LACTOENOS SB3 Direct (Laffort).

Conventional analysis. Conventional chemical analysis (e.g., ethanol, TA, pH, total anthocyanins, total phenols) was also performed using standard methods. Wine pH was obtained using standard methods (Reynolds and Wardle 1989). Wine TA was measured with a PC-Titrate autotitrator (Man-Tech Associates) to a pH 8.2 end point. Color intensity and hue were determined using a modified method provided by Mazza et al. (1999) and were calculated from absorbance values measured at 420 and 520 nm on an Ultrospec 2100 Pro UV-vis spectrophotometer (Biochrom Ltd.). Total anthocyanins were measured by the pH shift method (Fuleki and Francis 1968). Total phenols were determined according to the Folin-Ciocalteu micro method (Singleton and Rossi 1965, Waterhouse 2001).

Gas chromatography-mass spectrometry (GC-MS). Wines were analyzed using GC-MS with Gerstel thermal desorption technology according to previous methods (Bowen and Reynolds 2012, Moreno Luna et al. 2018). A 30-mL sample was taken from each wine treatment replicate immediately prior to bottling and was kept at 4°C in the presence of N2 inert gas until analysis. In duplicate, 100 μL of an internal standard, prepared with 10 μL of 98% 1-dodecanol (Sigma-Aldrich) in 10 mL of 100% ethyl alcohol (Commercial Alcohols, Inc.), was poured to the mark into a 10-mL volumetric flask followed by the addition of wine and then mixed. The prepared sample was transferred into a 10-mL Gerstel extraction vial followed by the addition of 10 mL of a saturated sodium chloride solution. A 10-mm stir bar (Twister, Gerstel, Inc.) coated with polydimethylsiloxane (0.5 mm film thickness) was added to the sample and stirred for 1 hr at 1000 g for extraction at room temperature. After extraction, the stir bar was removed, rinsed with Milli-Q water (Millipore) and dried out with lint free tissue, then placed in a 4-mL amber vial at 4°C until analysis during the same day. The stir bar was then inserted inside the extraction glass tube, which was thereafter placed inside the thermal desorption unit (TDU) attached to the GC-MS.

GC-MS conditions and conditioning of materials. An Agilent 6890N/5975B GC-MS equipped with a Gerstel TDU, cooled injection system, and programmable temperature vaporization was used. Columns were Agilent 19091S-433 HP-5MS 5% phenyl methylsiloxane, nominal length 30.0 m, nominal diameter 250.00 μm, nominal film thickness 0.25 μm; and J&W 122-7032 DB-WAX nominal length 30.0 m, nominal diameter 250.00 μm, nominal film thickness 0.25 μm. Instrument conditions were identical to Bowen and Reynolds (2012) and Moreno Luna et al. (2018). MS information included solvent delay: 3 min; scan acquisition method for identification compounds, low mass: 30; high mass: 400; threshold: 150; and selective ion monitoring/scan mode for quantification of aroma compounds. Stir bars used for extraction were conditioned every time before use to avoid any cross contamination. After analysis, each stir bar was kept overnight in a solution of 80:20 acetonitrile/methanol, allowed to dry, and then placed at 250°C for 2 hrs with a constant flow of nitrogen (N2) inert gas. All glass material was washed with Milli-Q water and methanol, then dried at 250°C for 1 hr.

Calibration compounds and odor activity values. Scan analysis reflected the presence of more than 100 volatile compounds in wines from both cultivars. For calibration purposes, 41 compounds were chosen for quantification (Table 1). Seven-point calibration curves were created for each compound consistent with the literature (Bowen and Reynolds 2012). Aroma standards were obtained from Acros Organics: 1-heptanol, phenylethanol, geraniol, and β-citronellol; Fluka: 2-phenylethyl acetate, linalool, terpinolene, δ-terpinene, and diethyl succinate; and Sigma-Aldrich: ethyl isobutyrate, ethyl hexanoate, ethyl heptanoate, ethyl octanoate, ethyl decanoate, isoamyl acetate, hexyl acetate, phenylethyl acetate, isoamyl alcohol, isobutyl alcohol, 1-hexanol, 1-octanol, α-terpineol, and β-damascenone.

View this table:
  • View inline
  • View popup
  • Download powerpoint
Table 1

Volatile standards for quantification of aroma compounds in Ontario Cabernet franc wines, 2017 to 2019. RT, retention time.

Model wine was used for calibration curves and prepared based on Bowen and Reynolds (2012), using 12% (v/v) of pure anhydrous ethanol (Commercial Alcohols, Inc.) diluted in Milli-Q water and 5 g/L of tartaric acid. The pH of the model wine was adjusted to 3.6 with 1N sodium hydroxide. Each aroma standard was diluted first in pure anhydrous ethanol at 1000 mg/L and kept at 4°C until analysis, then diluted at different concentrations in the model wine. Calibration samples were analyzed in SIM/SCAN mode using the same conditions as described previously with the use of the same internal standard. Odor activity values (OAVs) were calculated as a ratio between each concentration obtained by calibration versus their respective threshold. Thresholds were obtained from literature (Buttery et al. 1968, 1982, 1988, Ruth 1986, Takeoka et al. 1990, Etiévant 1991, Guth 1997, Ferreira et al. 2000, Plotto et al. 2004).

These data were used to generate concentrations of aroma compounds for comparative analysis. A list of these compounds and their aroma descriptors is found in Table 1. Methoxypyrazines were quantified by GC-MS using the methods of Kotseridis et al. (2008).

Statistical analysis. All data were analyzed using XL-Stat (Addinsoft). Data were also subjected to analysis of variance with mean separation by Duncan’s multiple range test, p < 0.05. All bar plot figures were prepared using the ‘gg-plot’ function in the ‘ggplot2’ package in an R environment (http://www.r-project.org/).

Results and Discussion

Effect of harvest strategy. 2017 season. In 2017, hand harvesting resulted in lowest concentrations of ethyl isobutyrate (plus MECH+BLR), ethyl nonoate, cis-linalool oxide (plus MECH and OPTI), trans-linalool oxide (plus MECH+OS), β-citral (plus MECH and OPTI), and cis- and trans-rose oxide (plus MECH, OPTI, and MECH+OS) (Figure 1 and Supplemental Table 1). Additionally, ethyl hexanoate was the lowest in MECH and MECH+BLR, isoamyl hexanoate was lowest in all treatments except HH, and α-ionone was lowest in MECH and MECH+BLR. Overall, the treatments led to the lowest concentrations as follows: HH (seven compounds), MECH (six compounds), MECH+BLR (four compounds), OPTI (five compounds), and MECH+OS (three compounds).

Figure 1
  • Download figure
  • Open in new tab
Figure 1

Effect of harvest technology on Ontario Cabernet franc aroma compounds, 2017. Control, hand harvested; OPTI, mechanically harvested using a Braud-New Holland optical sorting harvester; MECH+OPTI, conventional mechanical harvest followed by optical sorting; MECH+BLR, conventional mechanical harvest preceded by mechanical leaf removal; MECH, conventional mechanical harvest. Adapted with permission from Wang et al. (2020). Bars denoted by different letters are significantly different at p < 0.05, Duncan’s multiple range test.

2018 season. In 2018, hand harvesting resulted in lowest concentrations of β-damascenone, ethyl salicylate (plus OPTI and Gregoire), citronellol (plus Gregoire), cis-and trans-rose oxide (plus Gregoire), and eugenol (as well as Gregoire) (Figure 2 and Supplemental Table 2). Additionally, isobutyl acetate, isoamyl hexanoate, and nerol were reduced by Gregoire, and isopropylmethoxypyrazine was reduced by all treatments except HH. Overall, the treatments led to the lowest concentrations as follows: HH (six compounds), MECH (one compound), MECH+BLR (one compound), OPTI (two compounds), and Gregoire (nine compounds).

Figure 2
  • Download figure
  • Open in new tab
Figure 2

Effect of harvest technology on Ontario Cabernet franc aroma compounds, 2018. Control, hand harvested; Gregoire, mechanically harvested by a Gregoire GL8 harvester with optical sorting; OPTI, mechanically harvested using a Braud-New Holland optical sorting harvester; MECH+BLR, conventional mechanical harvest preceded by mechanical leaf removal; MECH, conventional mechanical harvest; IBMP, isobutyl methoxypyrazine; IPMP, isopropyl methoxypyrazine; SBPM, sec-butyl methoxypyrazine. Bars denoted by different letters are significantly different at p < 0.05, Duncan’s multiple range test.

2019 season. In the 2019 season, 27 of 41 compounds were affected by harvest strategy, including 11 esters, 12 terpenes, one norisoprenoid, and three miscellaneous compounds (Figure 3 and Supplemental Table 3). The treatments that led to reduced concentrations were as follows: HH (nine compounds; two esters, five terpenes, two miscellaneous compounds), MECH (eight compounds; five esters, three terpenes), MECH+BLR (10 compounds; four esters, five terpenes, α-ionone), OPTI (21 compounds; six esters, 11 terpenes, α-ionone, three miscellaneous compounds), Gregoire (10 compounds; two esters, six terpenes, two miscellaneous compounds), MECH+OS (22 compounds; seven esters, 11 ter penes, α-ionone, three miscellaneous compounds).

Figure 3
  • Download figure
  • Open in new tab
Figure 3
  • Download figure
  • Open in new tab
Figure 3

Effect of harvest technology on Ontario Cabernet franc aroma compounds, 2019. Control, hand harvested; Gregoire, mechanically harvested by a Gregoire GL8 harvester with optical sorting; OPTI, mechanically harvested using a Braud-New Holland optical sorting harvester; MECH+OPTI, conventional mechanical harvest followed by optical sorting; MECH+BLR, conventional mechanical harvest preceded by mechanical leaf removal; MECH, conventional mechanical harvest. Bars denoted by different letters are significantly different at p < 0.05, Duncan’s multiple range test.

When OAVs (concentration/threshold) are considered, a subset of the aforementioned compounds are considered of potential importance sensorially. Aliphatic compounds included four ethyl esters (with their 2017 to 2019 maximum OAV values): ethyl isobutyrate (OAV = 9640, 2140, 4520), ethyl hexanoate (OAV = 194, 67, 307), ethyl heptanoate (OAV = 4.6, 3.4, 6.2), and ethyl octanoate (OAV = 313, 109, 225); three acetate esters: isoamyl acetate (OAV = 48, 28, 84), isobutyl acetate (OAV = 1.7, 5.0, 8.5), and hexyl acetate (OAV = 7.8, 1.6, 4.2); and five alcohols: isobutanol (OAV = 23, 6.4, 21), isoamyl alcohol (OAV = 58, 10, 91), hexanol (OAV= 1.4, 0.3, 1.8), heptanol (OAV = 2.2, 1.8, 3.4), and phenylethanol (OAV = 20, 5.8, 19) (Figures 1 to 3 and Supplemental Tables 1 to 3). Among these compounds, all esters were responsive to harvest treatments in at least one of three seasons, and four (ethyl isobutyrate, ethyl hexanoate, isoamyl acetate, isobutyl acetate) were responsive in two seasons. None of the higher alcohols were responsive to harvest treatment. Although many terpenes were responsive to harvest treatments, only two were detected at concentrations that exceeded thresholds: geraniol (OAV = 1.5, 0.5, 0.6) and cis-rose oxide (OAV = 2.8, 2.2, 2.6), and of these, only cis-rose oxide was responsive to harvest treatment. Two norisoprenoids, β-damascenone (OAV= 433, 23, 291) and α-ionone (OAV= 9.2, 11, 19) were also responsive to harvest treatments and might therefore be assumed to play some role in floral taint.

Basic wine composition was affected to a limited degree by harvest technologies, and these strategies also varied between seasons (Supplemental Table 4). The highest pH was measured in the MECH+BLR wines (2017, 2018) and in HH (2019), whereas the lowest pH was measured in OPTI (2017, 2018) and MECH+OS (2019). Wine TA was highest in HH (2017, 2019) in addition to OPTI (2019), and lowest in MECH+BLR (2017, 2018); all remaining treatments shared highest position for TA in 2018 and lowest position in 2019. Wine color was highest in MECH+OS and lowest in MECH+BLR in 2017, but did not differ between treatments in 2018 and 2019. Anthocyanins were highest in OPTI (2017), HH (2018), and all but HH (2019), and were lowest in MECH+BLR (2017), OPTI (2018), and HH (2019). Wine phenols were highest in HH and lowest in OPTI (2018), but there were no treatment differences in the other two seasons. Ethanol was highest in MECH+BLR and lowest in OPTI (2017 only).

The high potential of grape leaves being integrated into the fermentation following mechanical harvesting poses a particular risk to the final wine composition (Noble et al. 1975, Wildenradt et al. 1975, Ward et al. 2015). Leaf blades (laminae) contain several volatile compounds that could potentially be extracted into wine, primarily C6 aldehydes and alcohols (2-hexanal; 2-hexen-1-ol; n-hexanol; 2-hexenol-1-ol; and hexa-2,4-dienal), terpenes (linalool, geraniol, citral, nerol, citronellol, α-terpineol, and myrcene), and norisoprenoids (β-damascenone, α-, and β-ionone) (Wildenradt et al. 1975, Gunata et al. 1986, Wang et al. 2020), although as shown in this study, many of these compounds are not necessarily detected at concentrations that exceed sensory threshold. The C6 compounds responsible for the “grassy” characteristics associated with grape leaves likely originate from lipoxygenase activity on the fatty acids in leaf cellular structures (Wildenradt et al. 1975). Petioles have high concentrations of monoterpenes, especially citronellol and geraniol (Gunata et al. 1986). The petiole may act as a storage instrument for free terpenes prior to transportation to other parts of the vine, or for utilization in metabolic pathways such as the mevalonate pathway, hence the high concentrations of terpenes in petioles compared to the laminae (Gunata et al. 1986).

Juice contact with MOG during fermentation results in extraction of several volatile grapevine compounds into the final wine (Ward et al. 2015, Suriano et al. 2016, Guerrini et al. 2018, Wang et al. 2020). The presence of increasing MOG during fermentation leads to wine with higher concentrations of several monoterpenes, including geraniol, linalool, nerol, β-citronellol, and α-terpineol, as well as other aroma compounds (Ward et al. 2015, Suriano et al. 2016, Guerrini et al. 2018, Wang et al. 2020). In the presence of high MOG concentrations, geraniol, linalool, and β-citronellol may be found at concentrations above their sensory detection thresholds, suggesting a potential effect on the perceived sensory profile (Ward et al. 2015). However, only two terpenes (geraniol and cis-rose-oxide) were found at OAV values >1 in this study. Higher alcohols and esters increase in the presence of stems (Suriano et al. 2016, Guerrini et al. 2018). Benzyl alcohol, eugenol, 1-hexanol, methyl salicylate, and ethyl salicylate can also increase as a result of MOG incorporation (Ward et al. 2015, Guerrini et al. 2018). However, contrary to other aroma compounds found in MOG treatments, methoxypyrazines can be reduced in the presence of high petiole concentrations (Ward et al. 2015). Ward et al. (2015) hypothesized that petioles may act to adsorb 3-isobutyl-2-methoxypyrazine from the fermenting wine.

It is noteworthy that very few studies have focused on the effects of mechanical harvesters with optical sorting capabilities, or optical sorting in general, and none have addressed the effects of incorporating frozen MOG into harvest loads on ultimate wine quality. Mechanical harvesting has been documented as affecting concentrations of numerous wine aroma compounds. Esters, including isoamyl acetate and hexyl acetate, were increased in mechanically harvested Montuni grapes (Arfelli et al. 2010). Several esters (ethyl 2-methylbutyrate, ethyl acetate, ethyl hexanoate, ethyl octanoate, isoamyl acetate) differed between harvest treatments in Pinot noir in California (Hendrickson et al. 2016), although as with this study, these compounds were not the main drivers of differences between hand harvest, mechanical harvest, and optical sorting treatment combinations. Higher alcohols have likewise been affected, including increases in hexanol and butanediol, plus reductions in isoamyl alcohol and phenylethyl alcohol (Arfelli et al. 2010). As with esters, Hendrickson et al. (2016) reported differences in several alcohols (benzyl alcohol, cis-2-hexen-1-ol, cis-3-hexen-1-ol, trans-2-hexen-1- ol, and trans-3-hexen-1-ol) between harvest treatments, but again as with the current study, none were the main foundations of treatment differences. Several organic acids have been reduced by mechanical harvesting, including isobutyric, isovaleric, and phenylacetic acids, whereas hexanoic, octanoic, and decanoic acids have been reported to increase, but their sensorial significance was not discussed (Arfelli et al. 2010).

Of greatest concern in this study were those compounds with low sensory thresholds—terpenes and norisoprenoids in particular—which were likely to be the main source of floral taint in wines produced from fruit mechanically harvested subsequent to killing frosts. In Australia, Cabernet Sauvignon wines produced from fruit with high MOG concentrations had geraniol, linalool, and β-citronellol concentrations above their detection thresholds, suggesting a potential sensory effect (Ward et al. 2015). Machine-harvested Pinot noir grapes in the Russian River Valley in California had higher concentrations of linalool, β-myrcene, α-terpinene, and β-damascenone, which were attributed to glycosidic hydrolysis due to berry damage during harvest or from wounding response-induced synthesis (Hendrickson et al. 2016). Other terpenes affected by mechanical pruning treatments included geraniol, nerol, nerol oxide, citronellol, and two sesquiterpenes (Hendrickson et al. 2016).

Impact of killing frost. Wines produced from fruit that had undergone a killing frost contained higher concentrations of 14 and eight compounds in 2018 in Cabernet franc and Cabernet Sauvignon, respectively (Table 2 and Supplemental Figure 1), and 17 and 13 compounds in 2019 in Cabernet franc and Cabernet Sauvignon, respectively (Table 3 and Supplemental Figure 2). Cabernet franc volatile compounds in most cases increased after a frost event; in 2018 these included four alcohols (isobutanol, isoamyl alcohol, hexanol, and phenylethanol), three esters (ethyl hexanoate, ethyl octanoate, and isoamyl acetate), five terpenes and norisoprenoids (linalool, geraniol, nerol, myrcene, and β-damascenone), and isopropyl methoxypyrazine (IPMP). In 2019, compounds that increased postfrost included octanol, eight esters, six terpenes (cis-linalool oxide, nerol, citronellol, α-terpineol, γ-terpinene, and eugenol), and β-damascenone. In Cabernet Sauvignon in 2018, only nerolidol increased after frost, whereas three esters (ethyl isobutyrate, ethyl heptanoate, and phenylethyl acetate) and four terpenes and norisoprenoids (citronellol, limonene, α-ionone, and β-ionone) decreased. In 2019, eight of 13 affected compounds increased after frost, including octanol, ethyl heptanoate, ethyl decanoate, cis-linalool oxide, citronellol, γ-terpinene, methyl salicylate, and ethyl salicylate. Ethyl isobutyrate, linalool, cis-rose oxide, trans-rose oxide, and terpinolene decreased. It is noteworthy that none of the methoxypyrazines were reduced by frost in either cultivar, contrary to the widespread anecdotal evidence in the Ontario industry.

View this table:
  • View inline
  • View popup
  • Download powerpoint
Table 2

Effect of preharvest frost on wine composition of Ontario Cabernet franc and Cabernet Sauvignon, 2018. Values are in µg/L unless otherwise specified. tr, trace.

View this table:
  • View inline
  • View popup
  • Download powerpoint
Table 3

Effect of preharvest frost on wine composition of Ontario Cabernet franc and Cabernet Sauvignon, 2019. Values are in µg/L unless otherwise specified.

The effect of frost on the ultimate basic wine composition was limited for both cultivars (Supplemental Table 5). For Cabernet franc, prefrost wines had slightly higher pH in 2018, but there were no other effects in that season and none in 2019. For Cabernet Sauvignon, there were no effects in 2018; freezing increased pH and reduced TA in 2019. No other effects were observed.

Studies into preharvest freezing of grapes intended for table wines are uncommon. Lan et al. (2016) reported that Beibinghong berries sampled in the last stages of maturity (which included freezing) were characterized by several terpenes (e.g., cis- and trans-linalool oxides and cis- and trans-rose oxides), norisoprenoids (cis- and trans-theaspirane and vitispirane A and B), and higher alcohols (e.g., heptenol, 2-octanol, and 1-butanol). In a related study involving comparisons with table wines produced from nonfrozen grapes, Beibinghong icewines made from frozen grapes were characterized by higher concentrations of volatile phenols, lactones, β-damascenone, phenylacetaldehyde, and diacetyl, versus corresponding dry wines made from fresh grapes (Lan et al. 2019). These differences were ascribed to “complex reactions induced by water loss and freezing-thawing events” during the on-vine dehydration and freezing processes. Delayed harvest of four cultivars in Ontario (Pinot gris, Riesling, Cabernet franc, and Cabernet Sauvignon), which involved brief freezing episodes for the red cultivars, resulted in substantial changes in most wine aroma compounds (Moreno Luna et al. 2018). Pinot gris and Riesling displayed increases in monoterpenes, norisoprenoids, esters, aldehydes, and alcohols in wines from late harvested fruit. Reduced concentrations of volatile acids and green odor compounds (e.g., 1-hexanol) with delayed harvest were also evident. Delayed harvest was also associated with the production of benzaldehyde, diethyl acetal, and higher concentrations of higher alcohols (e.g., isoamyl alcohol and nonanol), which could have been associated with preharvest desiccation rather than freezing. Cold maceration (without freezing) with automatic pump-over of Cabernet Sauvignon resulted in decreases in isobutanol and isopentanol and an increase in some esters (especially acetate esters) (Cai et al. 2014). Ethyl 2-hexenoate and diethyl succinate were decreased, and geranyl acetone was increased in both pump-over and punch-down fermentations, whereas β-damascenone increased by cold maceration in pump-over fermentations but decreased in punch-down fermentations.

Studies with harvest dates for icewine grapes suggest effects of multiple freezing events on aroma compounds. In an investigation of Ontario Vidal blanc and Riesling icewines, the highest concentrations for most aroma compounds were found in the latest of three harvest dates (16 of 24 for Vidal; 17 of 23 for Riesling) (Bowen and Reynolds 2015). The latest harvest date had the highest ethyl isobutyrate, ethyl 3-methylbutyrate, 1-hexanol, 1-octen-3-ol, 1-octanol, cis-rose oxide, nerol oxide, ethyl benzoate, ethyl phenylacetate, γ-nonalactone, and β-damascenone. The earliest harvest, which still had experienced at least one freezing episode, had the highest ethyl butyrate, ethyl hexanoate, linalool, 4-vinylguaiacol, and ethyl octanoate. However, there were no table wines produced in this study with which to compare the icewines to ascertain the effects of freezing. Similarly, Lutskova and Martirosyan (2021) compared early and later icewine harvests for four cultivars (Rkatsiteli, Telti kuruk, Marselan, and Moldova) and reported differences in aroma compounds; for example, Rkatsiteli icewine obtained from an early icewine harvest contained highest concentrations of geraniol, 1-octanol, and 2-phenyl acetate, while highest concentrations of ethyl hexanoate and ethyl octanoate were detected in the icewines from red cultivars Moldova and Marselan from a later harvest. However, once again, no wines produced from nonfrozen grapes were included for comparison.

Conclusions

These results suggest that specific harvest technologies can reduce MOG and its associated increases in undesirable aroma compounds, although seasonal differences may occur. It is apparent that mechanical harvesters with sorting capabilities can reduce MOG and consequently lower concentrations of compounds, such as terpenes, that may be associated with floral taints. In-winery optical sorting following conventional mechanical harvesting is likewise efficacious. A limited number of ethyl esters (ethyl isobutyrate, ethyl hexanoate, ethyl heptanoate, and ethyl octanoate); three acetate esters (isoamyl acetate, isobutyl acetate, and hexyl acetate); one terpene (cis-rose oxide), and two norisoprenoids (β-damascenone and α-ionone) were responsive to harvest treatments and also found at concentrations above sensory thresholds. Moreover, several esters, terpenes, and norisoprenoids increased following killing frost in hand-harvested grapes, suggesting that enhanced harvest technologies may not entirely overcome issues of floral taints in red wine cultivars.

Footnotes

  • Acknowledgments: We gratefully acknowledge the following partners: Ontario Grape & Wine Research Inc. and Ontario Centres of Excellence (funding); Andrew Peller Ltd. (wine fruit; optical sorting service 2019); Vineland Estates (optical sorting 2017); DIAM (corks); Lakeview Vineyard Equipment Inc. (Gregoire harvester); Thomas Oppenlaender (Braud-New Holland harvester).

  • Supplemental data is freely available with the online version of this article at www.ajevonline.org.

  • By downloading and/or receiving this article, you agree to the Disclaimer of Warranties and Liability. The full statement of the Disclaimers is available at http://www.ajevonline.org/content/proprietaryrights-notice-ajev-online. If you do not agree to the Disclaimers, do not download and/or accept this article.

  • Received January 2022.
  • Accepted January 2021.
  • Published online August 2022

This is an open access article distributed under the CC BY license (https://creativecommons.org/licenses/by/4.0/).

Literature Cited

    1. Ahmed EM ,
    2. Dennison RA ,
    3. Dougherty RH Shaw PE.
    1978. Flavor and odor thresholds in water of selected orange juice components. J Agric Food Chem 26:187-191.
    OpenUrl
  1. ↵
    1. Arfelli G ,
    2. Sartini E ,
    3. Bordini F ,
    4. Caprara C and
    5. Pezzi F.
    2010. Mechanical harvesting optimization and postharvest treatments to improve wine quality. J Int Sci Vigne Vin 44:101-115.
    OpenUrl
  2. ↵
    1. Bowen AJ and
    2. Reynolds AG.
    2012. Odor potency of aroma compounds in Riesling and Vidal blanc table wine and icewines by gas chromatography-olfactometry-mass spectrometry. J Agric Food Chem 60:2874-2883.
    OpenUrlPubMed
  3. ↵
    1. Bowen AJ and
    2. Reynolds AG.
    2015. Aroma compounds in Ontario Vidal and Riesling icewines. I. Effects of harvest date. Food Res Int 76:540-549.
    OpenUrl
  4. ↵
    1. Buttery RG ,
    2. Seifert RM ,
    3. Guadagni DG ,
    4. Black DR and
    5. Ling LC.
    1968. Characterization of some volatile constituents of carrots. J Agric Food Chem 16:1009-1015.
    OpenUrl
  5. ↵
    1. Buttery RG ,
    2. Seifert RM ,
    3. Ling LC ,
    4. Soderstrom EL ,
    5. Ogawa JM and
    6. Turnbaugh JG.
    1982. Additional aroma constituents of honeydew melon. J Agric Food Chem 30:1208-1211.
    OpenUrl
  6. ↵
    1. Buttery RG ,
    2. Turnbaugh JG and
    3. Ling LC.
    1988. Contribution of volatiles to rice aroma. J Agric Food Chem 36:1006-1009.
    OpenUrlCrossRef
  7. ↵
    1. Cai J ,
    2. Zhu B-Q ,
    3. Wang Y-H ,
    4. Lu L ,
    5. Lan Y-B ,
    6. Reeves MJ and
    7. Duan C-Q.
    2014. Influence of pre-fermentation cold maceration treatment on aroma compounds of Cabernet Sauvignon wines fermented in different industrial scale fermenters. Food Chem 154:217-229.
    OpenUrl
  8. ↵
    1. Christensen LP ,
    2. Kasimatis AN ,
    3. Kissler JJ ,
    4. Jensen F and
    5. Luvisi DA.
    1973. Mechanical harvesting of grapes for the winery. Calif Agric Exten Bull A XT-403, University of California, Davis.
  9. ↵
    1. Etiévant PXE.
    1991. Wine. In Volatile Compounds in Foods and Beverages. Maarse H. (ed.), pp. 483-546. Food Science and Technology, Dekker, New York.
  10. ↵
    1. Ferreira V ,
    2. López R and
    3. Cacho JF.
    2000. Quantitative determination of the odorants of young red wines from different grape varieties. J Sci Food Agric 80:1659-1667.
    OpenUrlCrossRef
  11. ↵
    1. Fuleki T and
    2. Francis FJ.
    1968. Quantitative methods for anthocyanins. 2. Determination of total anthocyanin and degradation index for cranberry juice. J Food Sci 33:78-83.
    OpenUrlCrossRef
  12. ↵
    1. Guerrini L ,
    2. Masella P ,
    3. Angeloni G ,
    4. Calamai L ,
    5. Spinelli S ,
    6. Di Blasi S and
    7. Parenti A.
    2018. Harvest of Sangiovese grapes: The influence of material other than grape and unripe berries on wine quality. Eur Food Res Technol 244:1487-1496.
    OpenUrlCrossRef
  13. ↵
    1. Gunata YZ ,
    2. Bayonove CL ,
    3. Baumes RL and
    4. Cordonnier RE.
    1986. Changes in free and bound fractions of aromatic components in vine leaves during development of muscat grapes. Phytochemistry 25:943-946.
    OpenUrlCrossRef
  14. ↵
    1. Guth H.
    1997. Quantitation and sensory studies of character impact odorants of different white wine varieties. J Agric Food Chem 45:3027-3032.
    OpenUrlCrossRef
  15. ↵
    1. Hendrickson DA and
    2. Oberholster A.
    2017. Review of the impact of mechanical harvesting and optical berry sorting on grape and wine composition. Catalyst 1:21-26.
    OpenUrl
  16. ↵
    1. Hendrickson DA ,
    2. Lerno LA ,
    3. Hjelmeland AK ,
    4. Ebeler SE ,
    5. Heymann H ,
    6. Hopfer H ,
    7. Block KL ,
    8. Brenneman CA and
    9. Oberholster A.
    2016. Impact of mechanical harvesting and optical berry sorting on grape and wine composition. Am J Enol Vitic 67:385-397.
    OpenUrlAbstract/FREE Full Text
  17. ↵
    1. Huang PJD ,
    2. Cash JN and
    3. Santerre CR.
    1988. Inf luence of stems, petioles and leaves on the phenolic content of Concord and Aurora blanc juice and wine. J Food Sci 53:173-175.
    OpenUrlCrossRef
  18. ↵
    1. Johnson SS.
    1977. Mechanical harvesting of wine grapes. Agricultural Economic Report 385, Economic Research Service, USDA, Washington, DC.
  19. ↵
    1. Joslin WS and
    2. Ough CS.
    1978. Cause and fate of certain C6 compounds formed enzymatically in macerated grape leaves during harvest and wine fermentation. Am J Enol Vitic 29:11-17.
    OpenUrlAbstract/FREE Full Text
  20. ↵
    1. Kilmartin PA and
    2. Oberholster A.
    2022. Grape harvesting and effects on wine composition. In Managing Wine Quality, 2nd Ed. Reynolds AG. (ed.), pp. 705-726. Woodhead, Cambridge, UK.
  21. ↵
    1. Kotseridis YS ,
    2. Spink M ,
    3. Brindle ID ,
    4. Blake AJ ,
    5. Sears M ,
    6. Chen X ,
    7. Soleas G ,
    8. Inglis D and
    9. Pickering GJ.
    2008. Quantitative analysis of 3-alkyl-2-methoxypyrazines in juice and wine using stable isotope labelled internal standard assay. J Chromatogr A 1190:294-301.
    OpenUrl
  22. ↵
    1. Lan Y ,
    2. Qian X ,
    3. Yang Z-J ,
    4. Xiang X-F ,
    5. Yang W-X ,
    6. Liu T ,
    7. Zhu B-Q ,
    8. Pan Q-H and
    9. Duan C-Q.
    2016. Striking changes in volatile profiles at sub-zero temperatures during over-ripening of ‘Beibinghong’ grapes in Northeastern China. Food Chem 212:172-182.
    OpenUrl
  23. ↵
    1. Lan Y et al.
    2019. Characterization and differentiation of key odor-active compounds of ‘Beibinghong’ icewine and dry wine by gas chromatography-olfactometry and aroma reconstitution. Food Chem 287:186-196.
    OpenUrl
    1. Leffingwell JC and
    2. Leffingwell D.
    1991. GRAS flavor chemicals—detection thresholds. Perfum Flavor 16:1-19.
    OpenUrl
  24. ↵
    1. Lutskova V and
    2. Martirosyan I.
    2021. Influence of harvest date and grape variety on sensory attributes and aroma compounds in experimental icewines of Ukraine. Fermentation 7:7. doi: https://doi.org/10.3390/fermentation7010007
    OpenUrl
  25. ↵
    1. Marais J.
    1983. Terpenes in the aroma of grapes and wines: A review. S Afr J Enol Vitic 4:49-58.
    OpenUrl
  26. ↵
    1. Matarese F ,
    2. Cuzzola A ,
    3. Scalabrelli G and
    4. D’Onofrio C.
    2014. Expression of terpene synthase genes associated with the formation of volatiles in different organs of Vitis vinifera . Phytochemistry 105:12-24.
    OpenUrlCrossRefPubMed
  27. ↵
    1. Mazza G ,
    2. Fukumoto L ,
    3. Delaquis P ,
    4. Girard B and
    5. Ewert B.
    1999. Anthocyanins, phenolics, and color of Cabernet Franc, Merlot, and Pinot noir wines from British Columbia. J Agric Food Chem 47:4009-4017.
    OpenUrlCrossRefPubMed
  28. ↵
    1. Morena Luna LH ,
    2. di Profio FD and
    3. Reynolds AG.
    2018. Impact of crop level and harvest date on composition of four wine grape cultivars in Ontario. II. Wine aroma compounds and sensory analysis. S Afric J Enol Vitic 39:246-270.
    OpenUrl
  29. ↵
    1. Noble AC ,
    2. Ough CS and
    3. Kasimatis AN.
    1975. Effect of leaf content and mechanical harvest on wine “quality”. Am J Enol Vitic 26:158-163.
    OpenUrlAbstract/FREE Full Text
  30. ↵
    1. Parenti A ,
    2. Spugnoli P ,
    3. Masella L ,
    4. Guerrini L ,
    5. Benedettelli S and
    6. Di Blasi S.
    2015. Comparison of grape harvesting and sorting methods on factors affecting the must quality. J Agric Eng 46:19-22.
    OpenUrlCrossRef
  31. ↵
    1. Pascual O ,
    2. González-Royo E ,
    3. Gil M ,
    4. Gómez-Alonso S ,
    5. García-Romero E ,
    6. Canals JM ,
    7. Hermosín-Gutíerrez I and
    8. Zamora F.
    2016. Influence of grape seeds and stems on wine composition and astringency. J Agric Food Chem 64:6555-6566.
    OpenUrl
  32. ↵
    1. Peterson R.
    1979. Most important machine picker feature may be night-time use. Calif Western States Grape Grower Dec, p. 14.
  33. ↵
    1. Petrucci VE and
    2. Siegfried R.
    1976. The extraneous matter in mechanically harvested wine grapes. Am J Enol Vitic 27:40-41.
    OpenUrlAbstract/FREE Full Text
  34. ↵
    1. Pezzi F ,
    2. Caprara C and
    3. Bordini F.
    2008. Transmission of impacts during mechanical grape harvesting and transportation. J Agric Eng 3:43-48.
    OpenUrl
  35. ↵
    1. Plotto A ,
    2. Margaría CA ,
    3. Goodner KL ,
    4. Goodrich R and
    5. Baldwin EA.
    2004. Odour and flavour thresholds for key aroma components in an orange juice matrix: terpenes and aldehydes. Flavour Fragr J 19:491-498.
    OpenUrl
  36. ↵
    1. Reynolds AG and
    2. Wardle DA.
    1989. Influence of fruit microclimate on monoterpene levels of Gewürztraminer. Am J Enol Vitic 40:149-154.
    OpenUrlAbstract/FREE Full Text
  37. ↵
    1. Ruth JH.
    1986. Odor thresholds and irritation levels of several chemical substances: A review. Am Ind Hygiene Assoc J 47:142-151.
    OpenUrl
  38. ↵
    1. Singleton VL and
    2. Rossi JA, Jr.
    1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144-158.
    OpenUrlAbstract/FREE Full Text
  39. ↵
    1. Spranger MI ,
    2. Clímaco MC ,
    3. Sun B ,
    4. Eiriz N ,
    5. Fortunato C ,
    6. Nunes A ,
    7. Leandro MC ,
    8. Avelar ML and
    9. Belchior AP.
    2004. Differentiation of red winemaking technologies by phenolic and volatile composition. Anal Chim Acta. 513:151-161.
    OpenUrl
  40. ↵
    1. Suriano S ,
    2. Alba V ,
    3. Di Gennaro D ,
    4. Basile T ,
    5. Tamborra M and
    6. Tarricone L.
    2016. Major phenolic and volatile compounds and their influence on sensorial aspects in stem-contact fermentation winemaking of Primitivo red wines. J Food Sci Technol 53:3329-3339.
    OpenUrl
  41. ↵
    1. Takeoka GR ,
    2. Flath RA ,
    3. Mon TR ,
    4. Teranishi R and
    5. Guentert M.
    1990. Volatile constituents of apricot (Prunus armeniacae). J Agric Food Chem 38:471-477.
    OpenUrlCrossRef
  42. ↵
    1. Wagener GWW.
    1980. The effect of mechanical harvesting on wines of Chenin blanc grapes in South Africa. Vitis 19:338-345.
    OpenUrl
  43. ↵
    1. Wang J ,
    2. Aubie E ,
    3. Lan Y ,
    4. Crombleholme M and
    5. Reynolds AG.
    2020. The impacts of frozen material-other-than-grapes (MOG) on aroma compounds of red wine varieties. Internet J Vitic Enol 9:1-9.
    OpenUrl
  44. ↵
    1. Ward SC ,
    2. Petrie PR ,
    3. Johnson TE ,
    4. Boss PK and
    5. Bastien SEP.
    2015. Unripe berries and petioles in Vitis vinifera cv. Cabernet Sauvignon fermentations affect sensory and chemical profiles. Am J Enol Vitic 66:435-443.
    OpenUrlAbstract/FREE Full Text
  45. ↵
    1. Waterhouse AL.
    2001. Determination of total phenolics. In Current Protocols in Food Analytical Chemistry. Wrolstad RE. (ed.), pp. I1.1.1-I1.1.8. John Wiley & Sons, Inc.
  46. ↵
    1. Wildenradt HL ,
    2. Christensen EN ,
    3. Stackler B ,
    4. Caputi A ,
    5. Slinkard K and
    6. Scutt K.
    1975. Volatile constituents of grape leaves. I. Vitis vinifera variety ‘Chenin Blanc’. Am J Enol Vitic 26:148-153
    OpenUrlAbstract/FREE Full Text
PreviousNext
Back to top

Vol 73 Issue 3

Issue Cover
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
Print
View full PDF
Email Article

Thank you for your interest in spreading the word on AJEV.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Effects of Frozen Materials Other Than Grapes on Red Wine Aroma Compounds. Impacts of Harvest Technologies
(Your Name) has forwarded a page to you from AJEV
(Your Name) thought you would like to read this article from the American Journal of Enology and Viticulture.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Open Access
Effects of Frozen Materials Other Than Grapes on Red Wine Aroma Compounds. Impacts of Harvest Technologies
Yibin Lan, Jiaming Wang, Emily Aubie, Marnie Crombleholme, Andrew Reynolds
Am J Enol Vitic.  2022  73: 142-155  ; DOI: 10.5344/ajev.2022.22006
Yibin Lan
1Center for Viticulture and Enology, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China;
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiaming Wang
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
3Postdoctoral Fellow. Present address: Labstat International Inc., Kitchener, ON;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Emily Aubie
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
4Postdoctoral Fellow. Present address: Andrew Peller Winery, 697 S Service Rd, Grimsby, ON L3M 4E8, Canada;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marnie Crombleholme
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
5Undergraduate thesis student. Present address: Quai du Vin Winery, St. Thomas, ON; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew Reynolds
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
6Former Professor. Present address: 613 Memorial Drive, Fenwick, ON L0S1C0; All research work was completed at Cool Climate Oenology & Viticulture Institute, Brock University.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: andrewreynolds2007@gmail.com

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Open Access
Effects of Frozen Materials Other Than Grapes on Red Wine Aroma Compounds. Impacts of Harvest Technologies
Yibin Lan, Jiaming Wang, Emily Aubie, Marnie Crombleholme, Andrew Reynolds
Am J Enol Vitic.  2022  73: 142-155  ; DOI: 10.5344/ajev.2022.22006
Yibin Lan
1Center for Viticulture and Enology, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China;
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiaming Wang
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
3Postdoctoral Fellow. Present address: Labstat International Inc., Kitchener, ON;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Emily Aubie
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
4Postdoctoral Fellow. Present address: Andrew Peller Winery, 697 S Service Rd, Grimsby, ON L3M 4E8, Canada;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marnie Crombleholme
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
5Undergraduate thesis student. Present address: Quai du Vin Winery, St. Thomas, ON; and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew Reynolds
2Cool Climate Oenology & Viticulture Institute, Brock University, St. Catharines, ON, L2S 3A1, Canada;
6Former Professor. Present address: 613 Memorial Drive, Fenwick, ON L0S1C0; All research work was completed at Cool Climate Oenology & Viticulture Institute, Brock University.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: andrewreynolds2007@gmail.com
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Save to my folders

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results and Discussion
    • Conclusions
    • Footnotes
    • Literature Cited
  • Figures & Data
  • Supplemental
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More from this TOC section

  • Diversity of Wild Yeasts During Spontaneous Fermentation of Wines from Local Grape Varieties in Turkey
  • Bioprotective Effect of Pichia kluyveri and Lactiplantibacillus plantarum in Winemaking Conditions
  • Vine Age Affects Vine Performance, Grape and Wine Chemical and Sensory Composition of cv. Zinfandel from California
Show more Research Articles

Similar Articles

AJEV Content

  • Current Volume
  • Archive
  • Best Papers
  • ASEV National Conference Technical Abstracts
  • Collections
  • Free Sample Issue
  • Print on Demand
  • Print on Demand

Information For

  • Authors
  • Open Access/Subscription Publishing
  • Submission
  • Subscribers
  • Permissions and Reproductions

Other

  • Home
  • About Us
  • Feedback
  • Help
  • Alerts
  • Catalyst
  • ASEV
asev.org

© 2023 American Society for Enology and Viticulture.  ISSN 0002-9254.

Powered by HighWire