Data supplements
Supplemental Table 1 Primers used for the quantification of gene expression levels by qRT-PCR.
Supplemental Table 2 Effect of cluster thinning and girdling (separated or combined) on the anthocyanin profiles (expressed as mg/g fresh weight) at harvest in 2016 and 2017.
Supplemental Figure 1 Daily average temperature (°C) and relative humidity (%) within the greenhouse of experimental vineyard at Shanghai Academy of Agricultural Sciences (Shanghai, China) throughout the ripening periods in 2016 (A) and 2017 (B).
Supplemental Figure 2 Expression levels of genes involved in sugar accumulation under cluster thinning and girdling (separated or combined) in two consecutive seasons. (A, B) VvAIN; (C, D) NI; (E, F) SS. Data presented are means ± SE (n = 3). Different letters indicate significant differences among treatments at the same date using Duncan’s multiple range test (p < 0.05).
Supplemental Figure 3 Comparison of skin coloration under cluster thinning and girdling (separated or combined) in 2017. (A) 57 DAA; (B) 71 DAA; (C) 85 DAA. CK, control; T1, girdling; T2, cluster thinning; T3, cluster thinning + girdling. The bar represents 1 cm.
Supplemental Figure 4 Effect of cluster thinning and girdling (separated or combined) on the concentration of five anthocyanin groups at harvest across two consecutive seasons. (A) 2016; (B) 2017. Cy, cyanidin derivatives; Dp, delphinidin derivatives; Mv, malvidin derivatives; Pn, peonidin derivatives; Pt, petunidin derivatives. Data presented are means ± SE (n = 3). Different letters indicate significant differences among treatments at the same date using Duncan’s multiple range test (p < 0.05).