Efficient Genetic Transformation of Vitis vinifera L. Mencía using a Hypervirulent Strain of Agrobacterium tumefaciens and qPCR Determination of Transgene Copy Number

Óscar Martínez; Elena Palomo-Ríos; Manuel Rey; María Victoria González

doi:10.5344/ajev.2024.24012

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Efficient Genetic Transformation of Vitis vinifera L. Mencía using a Hypervirulent Strain of Agrobacterium tumefaciens and qPCR Determination of Transgene Copy Number

View ORCID ProfileÓscar Martínez, View ORCID ProfileElena Palomo-Ríos, View ORCID ProfileManuel Rey, View ORCID ProfileMaría Victoria González

Am J Enol Vitic. 2024 75: 0750020 ; DOI: 10.5344/ajev.2024.24012

Óscar Martínez

1Departamento de Biología Vegetal y Ciencia del Suelo, Campus Universitario, Universidade de Vigo, 36310 Vigo, Spain;

4present address, Departamento de Biología Funcional, Universidad de Santiago de Compostela, Campus Sur, 15872 Santiago de Compostela, Spain.

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Elena Palomo-Ríos

2Departamento de Botánica y Fisiología Vegetal, Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM-UMA-CSIC), Universidad de Málaga, 29010 Málaga, Spain;

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Manuel Rey

1Departamento de Biología Vegetal y Ciencia del Suelo, Campus Universitario, Universidade de Vigo, 36310 Vigo, Spain;

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María Victoria González

3Departamento de Biología Funcional, Universidad de Santiago de Compostela, Campus Sur, 15872 Santiago de Compostela, Spain;

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For correspondence: mvictoria.gonzalez{at}usc.es

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vol. 75, article 0750020

DOI

https://doi.org/10.5344/ajev.2024.24012

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American Journal of Enology and Viticulture

History

Received March 2024
Accepted July 2024
Published online September 2024.

This is an open access article distributed under the CC BY 4.0 license.

Author Information

Óscar Martínez1,4,
Elena Palomo-Ríos2,
Manuel Rey1 and
María Victoria González3,*

¹Departamento de Biología Vegetal y Ciencia del Suelo, Campus Universitario, Universidade de Vigo, 36310 Vigo, Spain;
²Departamento de Botánica y Fisiología Vegetal, Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM-UMA-CSIC), Universidad de Málaga, 29010 Málaga, Spain;
³Departamento de Biología Funcional, Universidad de Santiago de Compostela, Campus Sur, 15872 Santiago de Compostela, Spain;
⁴present address, Departamento de Biología Funcional, Universidad de Santiago de Compostela, Campus Sur, 15872 Santiago de Compostela, Spain.

↵*Corresponding author (mvictoria.gonzalez{at}usc.es)

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Efficient Genetic Transformation of Vitis vinifera L. Mencía using a Hypervirulent Strain of Agrobacterium tumefaciens and qPCR Determination of Transgene Copy Number

View ORCID ProfileÓscar Martínez, View ORCID ProfileElena Palomo-Ríos, View ORCID ProfileManuel Rey, View ORCID ProfileMaría Victoria González

Am J Enol Vitic. 2024 75: 0750020 ; DOI: 10.5344/ajev.2024.24012

Óscar Martínez

1Departamento de Biología Vegetal y Ciencia del Suelo, Campus Universitario, Universidade de Vigo, 36310 Vigo, Spain;

4present address, Departamento de Biología Funcional, Universidad de Santiago de Compostela, Campus Sur, 15872 Santiago de Compostela, Spain.

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Elena Palomo-Ríos

2Departamento de Botánica y Fisiología Vegetal, Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM-UMA-CSIC), Universidad de Málaga, 29010 Málaga, Spain;

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Manuel Rey

1Departamento de Biología Vegetal y Ciencia del Suelo, Campus Universitario, Universidade de Vigo, 36310 Vigo, Spain;

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María Victoria González

3Departamento de Biología Funcional, Universidad de Santiago de Compostela, Campus Sur, 15872 Santiago de Compostela, Spain;

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ORCID record for María Victoria González
For correspondence: mvictoria.gonzalez{at}usc.es

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Efficient Genetic Transformation of Vitis vinifera L. Mencía using a Hypervirulent Strain of Agrobacterium tumefaciens and qPCR Determination of Transgene Copy Number

View ORCID ProfileÓscar Martínez, View ORCID ProfileElena Palomo-Ríos, View ORCID ProfileManuel Rey, View ORCID ProfileMaría Victoria González

Am J Enol Vitic. 2024 75: 0750020 ; DOI: 10.5344/ajev.2024.24012

Óscar Martínez

1Departamento de Biología Vegetal y Ciencia del Suelo, Campus Universitario, Universidade de Vigo, 36310 Vigo, Spain;

4present address, Departamento de Biología Funcional, Universidad de Santiago de Compostela, Campus Sur, 15872 Santiago de Compostela, Spain.

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ORCID record for Óscar Martínez

Elena Palomo-Ríos

2Departamento de Botánica y Fisiología Vegetal, Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM-UMA-CSIC), Universidad de Málaga, 29010 Málaga, Spain;

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ORCID record for Elena Palomo-Ríos

Manuel Rey

1Departamento de Biología Vegetal y Ciencia del Suelo, Campus Universitario, Universidade de Vigo, 36310 Vigo, Spain;

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ORCID record for Manuel Rey

María Victoria González

3Departamento de Biología Funcional, Universidad de Santiago de Compostela, Campus Sur, 15872 Santiago de Compostela, Spain;

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ORCID record for María Victoria González
For correspondence: mvictoria.gonzalez{at}usc.es

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Efficient Genetic Transformation of Vitis vinifera L. Mencía using a Hypervirulent Strain of Agrobacterium tumefaciens and qPCR Determination of Transgene Copy Number

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