Feeding by Adult Spotted Lanternfly Affects Carbon Allocation Postinfestation in Young Grapevines

Supplemental Figure 1   Examples of 3-yr-old container-grown Cabernet franc grapevines netted with insect exclusion netting (AgFabric, WellCo Industries, Inc.). The image was taken on 25 Aug 2022. Grapevines were exposed to zero spotted lanternflies (SLFs) (Control; 0 SLFs/vine; n = 10) or high numbers of adult SLFs (SLF; 15 to 20 SLFs/shoot, or 45 to 60 SLFs/vine; n = 10) from 25 Aug to 30 Sept 2022.

Supplemental Figure 2   Single-leaf carbon assimilation, transpiration, and stomatal conductance (measured on 29 Sept 2022) of 3-yr-old, container-grown Cabernet franc grapevines exposed to zero spotted lanternflies (SLFs) (Control; 0 SLFs/vine; n = 10) or high numbers of adult SLFs (SLF; 15 to 20 SLFs/shoot, or 45 to 60 SLFs/vine; n = 10) from 25 Aug to 30 Sept 2022, a day before application of ¹³CO₂.

Appendix 1  Method for analysis of ¹³C content in grapevine tissues, modified from previously published protocols.

Appendix 2  Method for extraction and analysis of soluble sugars and starch in grapevine tissues, and modifications made to previously published protocols.

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