Abstract
Knowledge of total non-structural carbohydrates in plant material is a useful tool in understanding the effects of environmental conditions and vineyard management decisions on grapevine physiology. There are a wide range of techniques described in the literature, varying in their degree of sophistication, with few having been validated in grapevines. We describe the miniaturization of a simple extraction method and microplate based colorimetric assay for the measurement of non-structural carbohydrates in a range of grapevine tissues. The method provides quantitative results for soluble carbohydrates as well as starch in samples containing as little as 20 μg of carbohydrate.
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