Skip to main content
Advertisement

Main menu

  • Home
  • Content
    • Current Volume
    • AJEV and Catalyst Archive
    • Best Papers
    • ASEV National Conference Technical Abstracts
    • Print on Demand
  • Information For
    • Authors
    • Open Access Publishing
    • AJEV Preprint and AI Software Policy
    • Submission
    • Subscribers
      • Proprietary Rights Notice for AJEV Online
    • Permissions and Reproductions
  • About Us
  • Feedback
  • Alerts
  • Help
  • Login
  • ASEV MEMBER LOGIN

User menu

  • Log in

Search

  • Advanced search
American Journal of Enology and Viticulture
  • Log in
  • Follow ajev on Twitter
  • Follow ajev on Linkedin
American Journal of Enology and Viticulture

Advanced Search

  • Home
  • Content
    • Current Volume
    • AJEV and Catalyst Archive
    • Best Papers
    • ASEV National Conference Technical Abstracts
    • Print on Demand
  • Information For
    • Authors
    • Open Access Publishing
    • AJEV Preprint and AI Software Policy
    • Submission
    • Subscribers
    • Permissions and Reproductions
  • About Us
  • Feedback
  • Alerts
  • Help
  • Login
  • ASEV MEMBER LOGIN
Research Article

A Novel Grape Downy Mildew Resistance Locus from Vitis rupestris

Gaurab Bhattarai, Anne Fennell, Jason P. Londo, Courtney Coleman, Laszlo G. Kovacs
Am J Enol Vitic.  2020  : ajev.2020.20030  ; DOI: 10.5344/ajev.2020.20030
Gaurab Bhattarai
1Department of Biology, Missouri State University, Springfield, MO 65897
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Anne Fennell
2Department of Agronomy, Horticulture & Plant Science, South Dakota State University, Brookings, SD 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Jason P. Londo
3Grape Genetics Research Unit, USDA-Agriculture Research Service, Geneva, NY 14456
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Courtney Coleman
1Department of Biology, Missouri State University, Springfield, MO 65897
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
Laszlo G. Kovacs
1Department of Biology, Missouri State University, Springfield, MO 65897
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Find this author on ADS search
  • Find this author on Agricola
  • Search for this author on this site
  • For correspondence: Laszlokovacs@missouristate.edu
  • Article
  • Figures & Data
  • Supplemental
  • Info & Metrics
  • PDF
Loading

Data supplements

  • Supplemental Data  R-script to plot recombination fraction and logarithm of odds (LOD) threshold. ##Broman KW, Wu H, Sen S, Churchill GA (2003) R/qtl: quantitative trait locus (QTL) mapping in experimental crosses. Bioinformatics 19:889-890.

    Supplemental Figure 1   Leaf disk assay based on OIV descriptors 452-1 (International Organization of Vine and Wine). Panels A, B, C, D, and E show examples of very low, low, medium, high, and very high level of resistance, where 50% or more, ~20%, 10%, >5%, and none of the leaf disk area was covered by sporangiophores, respectively, seven days postinoculation. Scores assigned to the levels of resistance depicted in panels A, B, C, D, and E were 1, 3, 5, 7, and 9, respectively.

    • SupplementalData_SupplementalFigure1.pdf
  • Supplemental Figure 2   Integrated linkage map.

    • SupplementalFigure2.pdf
  • Supplemental Figure 3   Synteny between genetic distance and physical distance in Vitis rupestris, Vitis riparia, and integrated maps.

    Supplemental Figure 4  Pairwise recombination fractions and logarithm of odds (LOD) plots for parental maps. Pairwise recombination fractions and LOD scores in (A) Vitis rupestris and (B) Vitis riparia linkage groups. Yellow and blue coloring below the identity line represent low and high crossover frequency regions, respectively. Yellow and blue coloring above the identity line represent high and low LOD values, respectively. The top X- and right Y-axes are numbered according to linkage groups; the bottom X- and left Y-axes indicate the position of markers in the 19 linkage groups.

    Supplemental Figure 5  Flower sex-determining quantitative trait locus (QTL) on chromosome 2. Maleness-determining locus on chromosome 2 in the integrated map. Right panel shows a significant logarithm of odds (LOD) peak (p < 0.05) with corresponding tightly linked marker within the maleness-determining QTL region (QTL.Sex) on chromosome 2. The horizontal black dashed-dotted line represents genome-wide LOD threshold (number of permutations = 1000) at p = 0.05. Marker in red color represents a marker with the largest LOD value.

    Supplemental Figure 6   Phylogenetic relatedness of downy mildew strain MO-1. Position of Plasmopara viticola strain MO-1 in the phylogeny of P. viticola cryptic species. Phylogenetic tree was constructed with the 235-bp long internal transcribed spacer-1 (ITS-1) nucleotide sequence of the 5.8S ribosomal RNA gene (GeneBank accession: JF897779; Rouxel et al. 2014).

    Supplemental Figure 7   Frequency distribution of F1 progeny for disease resistance trait. Disease resistance as measured by leaf area covered by sporangiophores under (A) greenhouse and (B) in vitro conditions.

    • SupplementalFigures_3-7.pdf
  • Supplemental Table 1   Vitis rupestris map: Markers, ID and its segregation type, phase information, classification, genetic position, corresponding linkage group and genotypes across individuals. Number of markers = 1177; number of F1 individuals = 294.

    • SupplementalTable_1.xlsx
  • Supplemental Table 2   Vitis riparia map: Markers, ID and its segregation type, phase information, classification, genetic position, corresponding linkage group and genotypes across individuals. Number of markers = 1115; number of F1 individuals = 294.

    • SupplementalTable_2.xlsx
  • Supplemental Table 3  Integrated map: Markers, ID and its segregation type, phase information, classification, genetic position, corresponding linkage group and genotypes across individuals. Number of markers = 2583; number of F1 individuals = 294.

    • SupplementalTable_3.xlsx
  • Supplemental Table 4   In vitro disease coverage. Mean and standard deviation of scores for leaf disks calculated for each genotype.

    Supplemental Table 5   Logarithm of odds (LOD) values from interval mapping across Vitis rupestris chromosome 10 for downy mildew resistance under greenhouse and in vitro conditions (LOD Threshold: 4.8) and the list genotyping-by-sequencing (GBS) markers, genetic positions, and corresponding Vitis vinifera RefSeq (12X.v3 assembly, Canaguier et al. 2017) coordinates within the 2-LOD interval of Rpv28.1 (dark gray highlight) and Rpv28.2 (light gray highlight).

    • SupplementalTable_4-5.pdf
  • Supplemental Table 6   Predicted genes within the 2-LOD interval of Rpv28.1 as inferred from the corresponding Vitis vinifera RefSeq (12X.v3 assembly, Canaguier et al 2017).

    • SupplementalTable_6.xlsx
  • Supplemental Table 7   SSR markers within the 2-LOD interval of Rpv28.2.

    • SupplementalTable_7.pdf
Next
Back to top
View full PDF
Email Article

Thank you for your interest in spreading the word on AJEV.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
A Novel Grape Downy Mildew Resistance Locus from Vitis rupestris
(Your Name) has forwarded a page to you from AJEV
(Your Name) thought you would like to read this article from the American Journal of Enology and Viticulture.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
You have accessRestricted access
A Novel Grape Downy Mildew Resistance Locus from Vitis rupestris
Gaurab Bhattarai, Anne Fennell, Jason P. Londo, Courtney Coleman, Laszlo G. Kovacs
Am J Enol Vitic.  2020  ajev.2020.20030  ; DOI: 10.5344/ajev.2020.20030
Gaurab Bhattarai
1Department of Biology, Missouri State University, Springfield, MO 65897
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anne Fennell
2Department of Agronomy, Horticulture & Plant Science, South Dakota State University, Brookings, SD 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jason P. Londo
3Grape Genetics Research Unit, USDA-Agriculture Research Service, Geneva, NY 14456
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Courtney Coleman
1Department of Biology, Missouri State University, Springfield, MO 65897
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laszlo G. Kovacs
1Department of Biology, Missouri State University, Springfield, MO 65897
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: Laszlokovacs@missouristate.edu

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
You have accessRestricted access
A Novel Grape Downy Mildew Resistance Locus from Vitis rupestris
Gaurab Bhattarai, Anne Fennell, Jason P. Londo, Courtney Coleman, Laszlo G. Kovacs
Am J Enol Vitic.  2020  ajev.2020.20030  ; DOI: 10.5344/ajev.2020.20030
Gaurab Bhattarai
1Department of Biology, Missouri State University, Springfield, MO 65897
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anne Fennell
2Department of Agronomy, Horticulture & Plant Science, South Dakota State University, Brookings, SD 57007
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jason P. Londo
3Grape Genetics Research Unit, USDA-Agriculture Research Service, Geneva, NY 14456
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Courtney Coleman
1Department of Biology, Missouri State University, Springfield, MO 65897
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laszlo G. Kovacs
1Department of Biology, Missouri State University, Springfield, MO 65897
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: Laszlokovacs@missouristate.edu
del.icio.us logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Save to my folders

Jump to section

  • Article
  • Figures & Data
  • Supplemental
  • Info & Metrics
  • PDF

Related Articles

Cited By...

More from this TOC section

  • Diversity of Wild Yeasts During Spontaneous Fermentation of Wines from Local Grape Varieties in Turkey
  • Bioprotective Effect of Pichia kluyveri and Lactiplantibacillus plantarum in Winemaking Conditions
  • Vine Age Affects Vine Performance, Grape and Wine Chemical and Sensory Composition of cv. Zinfandel from California
Show more Research Article

Similar Articles

AJEV Content

  • Current Volume
  • Archive
  • Best Papers
  • ASEV National Conference Technical Abstracts
  • Print on Demand

Information For

  • Authors
  • Open Access Publishing
  • AJEV Preprint and AI Software Policy
  • Submission
  • Subscribers
  • Permissions and Reproductions

Other

  • Home
  • About Us
  • Feedback
  • Help
  • Alerts
  • ASEV
asev.org

© 2025 American Society for Enology and Viticulture.  ISSN 0002-9254.

Powered by HighWire