TY - JOUR T1 - Grapevine Non-<em>Vinifera</em> Genetic Diversity Assessed by SSR Markers as a Starting-Point for New Rootstock Breeding Programs JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. DO - 10.5344/ajev.2019.18054 SP - ajev.2019.18054 AU - Daniele Migliaro AU - Gabriella De Lorenzis AU - Giovambattista Simone Di Lorenzo AU - Barbara De Nardi AU - Massimo Gardiman AU - Osvaldo Failla AU - Lucio Brancadoro AU - Manna Crespan Y1 - 2019/06/07 UR - http://www.ajevonline.org/content/early/2019/06/05/ajev.2019.18054.abstract N2 - Microsatellite markers are a valuable tool to facilitate the management of germplasm collections and access genetic diversity. This study reports the genetic characterization of a large collection of 379 rootstocks and other non-vinifera accessions maintained at the University of Milan (Italy). Accessions were genotyped with 22 highly polymorphic microsatellite markers (including the nine ‘international’ loci used for grapevine identification, three VMC, three VrZAG and seven VChr loci), but only 17 loci were retained for cultivar identification, to investigate genetic diversity, for pedigree analysis, to infer population structure and design a core collection. Two hundred and thirty-two unique genotypes were identified. The allelic profiles of sixty-nine rootstocks were confirmed according to the literature and databases, while the profiles of 44 rootstocks were proposed for the first time. Pedigree analysis highlighted 77 parent-offspring (PO) trios and 43 PO relationships, some of them already known and others new. Analysis of the genetic structure showed a more likely number of three ancestral groups, with a high percentage of admixed samples. A structure based on the genetic background of genotypes was not observed. A core collection of 70 genotypes captured 100% of the entire number (373) of detected alleles. Most of these genotypes were unidentified or poorly characterized. The information provided in this paper could assist breeders in their efforts to exploit still unexplored individuals useful for long-term breeding plans. ER -