PT - JOURNAL ARTICLE AU - V. L. Singleton AU - Joseph A. Rossi, Jr. TI - Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents AID - 10.5344/ajev.1965.16.3.144 DP - 1965 Jan 01 TA - American Journal of Enology and Viticulture PG - 144--158 VI - 16 IP - 3 4099 - http://www.ajevonline.org/content/16/3/144.short 4100 - http://www.ajevonline.org/content/16/3/144.full SO - Am J Enol Vitic.1965 Jan 01; 16 AB - Several details of the assay of total phenolic substances have been investigated and an improved procedure developed. The improvements include the use of Folin-Ciocalteu reagent rather than the Folin-Denis reagent, gallic acid as a reference standard, and a more reproducible time-temperature color development period. The values obtained are less subject to variation and interference from several nonphenols, yet are directly comparable to the "tannin" values obtained by the previously standard method.