TY - JOUR T1 - Low Expression of Flavonoid 3′,5′-Hydroxylase (<em>F3</em>′,<em>5</em>′<em>H</em>) Associated with Cyanidin-based Anthocyanins in Grape Leaf JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 362 LP - 367 DO - 10.5344/ajev.2009.60.3.362 VL - 60 IS - 3 AU - Hironori Kobayashi AU - Shunji Suzuki AU - Fumiko Tanzawa AU - Tsutomu Takayanagi Y1 - 2009/09/01 UR - http://www.ajevonline.org/content/60/3/362.abstract N2 - The aim of this study was to clarify anthocyanin composition and transcription profiles of anthocyanin-synthesis-related genes in grape (Vitis vinifera L.) leaf during development. Berry skin accumulated both cyanidin-based anthocyanins and delphinidin-based anthocyanins during development and showed up-regulation of flavonoid 3′,5′-hydroxylase gene (F3′,5′H) expression. Grape leaf, in contrast, accumulated a large amount of cyanidin-based anthocyanins during development. This result was supported by the low expression of F3′,5′H in developing grape leaf. The low expression of F3′,5′H and the low level of delphinidin-based anthocyanins in grape leaf were consistent in green-yellow-, purple-, and black-colored skin cultivars. Flavonoid 3′-hydroxylase gene (F3′H) expression increased in grape leaf during development. Since F3′,5′H regulates the synthesis of delphinidin-based anthocyanins and F3′H regulates the synthesis of cyanidin-based anthocyanins in plants, it is possible that grape leaf accumulates large amount of cyanidin-based anthocyanins through the cyanidin synthesis pathway regulated by F3′H. ER -