RT Journal Article SR Electronic T1 Spectrophotometric Determination of Ethanol in Wine JF American Journal of Enology and Viticulture JO Am J Enol Vitic. FD American Society for Enology and Viticulture SP 160 OP 165 DO 10.5344/ajev.1968.19.3.160 VO 19 IS 3 A1 Caputi, Arthur A1 Ueda, Masao A1 Brown, Thomas YR 1968 UL http://www.ajevonline.org/content/19/3/160.abstract AB A variation of the micro-dichromate method for the determination of ethanol in wine was devised. Reproducible pipetting of a 1-ml sample was achieved through the use of an automatic diluter. Distillation of the sample into an acidic potassium dichromate solution in a 50-ml volumetric flask permitted simple dilution to volume after heating 20 minutes at 60° C. Percent alcohol by volume was then determined from a direct digital concentration spectrophotometer af 600 mµ after standardization with a known ethanol-water mixture, similarly distilled and reacted. The method eliminates the need for a standard ferrous ammonium sulfate solution as a titrant and the normal indicator solution as well as the difficulties encountered with a 1-ml volumetric pipette.