PT  - JOURNAL ARTICLE
AU  - Gregg A. Cremer
AU  - Roger Boulton
TI  - The Hydrolysis of Gelatin by an Immobilized Acid Protease. I. Immobilization and Hydrolysis Kinetics
AID  - 10.5344/ajev.1981.32.1.14
DP  - 1981 Jan 01
TA  - American Journal of Enology and Viticulture
PG  - 14--17
VI  - 32
IP  - 1
4099  - http://www.ajevonline.org/content/32/1/14.short
4100  - http://www.ajevonline.org/content/32/1/14.full
SO  - Am J Enol Vitic.1981 Jan 01; 32
AB  - An acid protease from Aspergillus saitoi was immobilized on agarose beads of approximately 100 micrometers diameter. Solutions of gelatin were prepared in a saturated potassium bitartrate buffer and contacted with the immobilized enzyme. The Michaelis-Menten constant was determined to be 7.8 x 10-5 M at 25°C and pH of 3.5.The maximum velocity of hydrolysis corrected for bed voidage was found to be 6.8 x 10-7 M per second under the same conditions.