PT - JOURNAL ARTICLE AU - J. R. Perez AU - W. M. Kliewer TI - Influence of Light Regime and Nitrate Fertilization on Nitrate Reductase Activity and Concentrations of Nitrate and Arginine in Tissues of Three Cultivars of Grapevines AID - 10.5344/ajev.1982.33.2.86 DP - 1982 Jan 01 TA - American Journal of Enology and Viticulture PG - 86--93 VI - 33 IP - 2 4099 - http://www.ajevonline.org/content/33/2/86.short 4100 - http://www.ajevonline.org/content/33/2/86.full SO - Am J Enol Vitic.1982 Jan 01; 33 AB - Three grapevine (Vitis vinifera L.) cultivars, Chardonnay, Malbec, and Zinfandel were grown in sand culture at four different levels of solar radiation (100%, 50%, 28%, and 8% of full sunlight) in combination with three different levels of nitrogen (16, 4, and 1 mM nitrate).Nitrate in petioles and blades was inversely related to light availability and directly related to nitrogen level. Dry matter per unit length of shoot growth, total cane dry wt./vine and nitrate reductase (NR, the enzyme that catalyzes the reduction of NO3 to NO2) activity of leaf blades were related directly to light intensity, decreasing sharply under reduced solar radiation. The influence of solar radiation on the level of arginine was variable. The concentration of arginine in dormant cane tissues depended mainly on cultivar and on the concentration of nitrogen in the nutrient solution.Nitrate reductase activity was related inversely to petiole nitrate concentration and correlated highly with weight per centimenter of cane. The level of nitrate accumulated in petioles and leaf blades was modulated, at least in part, by the activity of nitrate reductase.It is concluded that where climate is variable, especially during the bloom sampling period, petiole nitrate may not reflect the true nitrogen status of vineyards.