@article {Rajasekaran108, author = {K. Rajasekaran and Michael G. Mullins}, title = {The Origin of Embryos and Plantlets from Cultured Anthers of Hybrid Grapevines}, volume = {34}, number = {2}, pages = {108--113}, year = {1983}, doi = {10.5344/ajev.1983.34.2.108}, publisher = {American Journal of Enology and Viticulture}, abstract = {Callus derived from cultured anthers of Gloryvine (Vitis vinifera L. x V. rupestris Scheele) contained both haploid (n=19) and diploid (2n=38) cells, but all the plantlets produced in vitro were diploid. The presence of haploid cells in callus derived from anthers was further indicated by the occurrence of nuclei with the 1C-DNA level. Haploid cells were not found in callus from internode explants. Multinucleate pollen grains were observed, but there was strong anatomical evidence that the anther-callus originated primarily from somatic cells of the anthers wall and the connective. There was considerable variation among anther-derived plants in leaf morphology. Progeny of selfed anther-derived plants exhibited segregation for leaf characters indicating heterozygosity and somatic origin. Isoenzyme patterns for indophenol oxidase, leucine amino peptidase and catechol oxidase in anther-derived plants and in the original genotype from which the cultures were established were indistinguishable.}, issn = {0002-9254}, URL = {https://www.ajevonline.org/content/34/2/108}, eprint = {https://www.ajevonline.org/content/34/2/108.full.pdf}, journal = {American Journal of Enology and Viticulture} }