TY - JOUR T1 - The Origin of Embryos and Plantlets from Cultured Anthers of Hybrid Grapevines JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 108 LP - 113 DO - 10.5344/ajev.1983.34.2.108 VL - 34 IS - 2 AU - K. Rajasekaran AU - Michael G. Mullins Y1 - 1983/01/01 UR - http://www.ajevonline.org/content/34/2/108.abstract N2 - Callus derived from cultured anthers of Gloryvine (Vitis vinifera L. x V. rupestris Scheele) contained both haploid (n=19) and diploid (2n=38) cells, but all the plantlets produced in vitro were diploid. The presence of haploid cells in callus derived from anthers was further indicated by the occurrence of nuclei with the 1C-DNA level. Haploid cells were not found in callus from internode explants. Multinucleate pollen grains were observed, but there was strong anatomical evidence that the anther-callus originated primarily from somatic cells of the anthers wall and the connective. There was considerable variation among anther-derived plants in leaf morphology. Progeny of selfed anther-derived plants exhibited segregation for leaf characters indicating heterozygosity and somatic origin. Isoenzyme patterns for indophenol oxidase, leucine amino peptidase and catechol oxidase in anther-derived plants and in the original genotype from which the cultures were established were indistinguishable. ER -