RT Journal Article
SR Electronic
T1 A Rapid HPLC Method for Separation and Determination of Major Organic Acids in Grape Musts and Wines
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 151
OP 155
DO 10.5344/ajev.1987.38.2.151
VO 38
IS 2
A1 A. Schneider
A1 V. Gerbi
A1 M. Redoglia
YR 1987
UL http://www.ajevonline.org/content/38/2/151.abstract
AB An isocratic high performance liquid chromatographic (HPLC) method was developed for the separation and quantitative analysis of the major carboxylic acids of grape musts and wines such as citric, tartaric, malic, lactic, and acetic acids. By using an Aminex HPX87-H, a strong cation exchange resin column eluted with dilute sulfuric acid, and monitoring with uv at 210 nm, sample preparation is reduced to simple membrane filtration for white wines or musts and to the removal of phenolic compounds employing disposable reverse phase cartridges for red wines. The use of mobile phase at a different pH for grape must and wine sample analysis allows the separation of compounds such as malic acid and fructose that normally coelute. The results of the HPLC analyses were compared with data obtained by other methods in a range of white wine, red wine, and must samples.