RT Journal Article
SR Electronic
T1 High Performance Liquid Chromatography-Diode Array Spectroscopy Identification of Anthocyanins of <em>Vitis vinifera</em> variety Tempranillo
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 227
OP 233
DO 10.5344/ajev.1988.39.3.227
VO 39
IS 3
A1 Hebrero, E.
A1 Santos-Buelga, C.
A1 Rivas-Gonzalo, J. C.
YR 1988
UL http://www.ajevonline.org/content/39/3/227.abstract
AB High performance liquid chromatography (HPLC) and diode array spectroscopy (DAS) were applied simultaneously for the separation, identification, and quantification of the anthocyanins of Vitis vinifera variety Tempranillo. Use of diode array detector (DAD) permitted simultaneous recording of the chromatographic analysis at several wavelengths and the visible-ultraviolet (v-uv) spectra of the different anthocyanins separated by HPLC. Using these techniques and on the basis of their elution order and spectroscopic properties, 15 anthocyanins were identified: the 3-monoglucosides of delphinidin, cyanidin, petunidin, peonidin, and malvidin and the derivatives of these five compounds acylated with acetic and p-coumaric acids. Acid and alkaline hydrolysis of both the anthocyanins isolated and the total anthocyanin extract obtained from the grapes confirm the identities of these compounds.