TY - JOUR T1 - Determination of Major Carboxylic Acids in Wine by an Optimized HPLC Method with Linear Gradient Elution JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 289 LP - 294 DO - 10.5344/ajev.1990.41.4.289 VL - 41 IS - 4 AU - R. M. Marcé AU - M. Calull AU - F. Borrull AU - F. X. Rius Y1 - 1990/01/01 UR - http://www.ajevonline.org/content/41/4/289.abstract N2 - An optimized method to determine carboxylic acids in wine based on pre-column derivatization with phenacyl bromide is reported. The determination of the different acids has been carried out using a linear gradient elution involving water and methanol. The method has been optimized by the Simplex method using a modification of CRF (chromatographic response factor) as the criterion for selectivity optimization. After validation, the method was applied to determine carboxylic acids in red and white wines and grape must of the Tarragona region. ER -