PT  - JOURNAL ARTICLE
AU  - Cauré Portugal
AU  - Fernanda Ruiz-Larrea
TI  - Comparison of Specific Real-Time PCR and Conventional Culture for Detection and Enumeration of <em>Brettanomyces</em> in Red Wines
AID  - 10.5344/ajev.2012.12047
DP  - 2013 Mar 01
TA  - American Journal of Enology and Viticulture
PG  - 139--145
VI  - 64
IP  - 1
4099  - http://www.ajevonline.org/content/64/1/139.short
4100  - http://www.ajevonline.org/content/64/1/139.full
SO  - Am. J. Enol. Vitic.2013 Mar 01; 64
AB  - Brettanomyces/Dekkera is considered as one of the main causes of microbial spoilage and sensory deviations of red wines. This work compares the sensitivity and effectiveness of conventional microbiological culture and real-time polymerase chain reaction (Q-PCR) methods for Brettanomyces/Dekkera detection and quantification and demonstrates a positive correlation between both methods. Moreover, an improved DNA extraction protocol enabled quantification of Brettanomyces/Dekkera cells by Q-PCR down to 20 cells/mL in turbid wines in a total of 324 red wine samples. The conventional culture analysis is time-consuming but has lower cost than Q-PCR, and it is simple and efficient in quantifying viable Brettanomyces/Dekkera cells.