RT Journal Article SR Electronic T1 Comparison of Specific Real-Time PCR and Conventional Culture for Detection and Enumeration of Brettanomyces in Red Wines JF American Journal of Enology and Viticulture JO Am. J. Enol. Vitic. FD American Society for Enology and Viticulture SP 139 OP 145 DO 10.5344/ajev.2012.12047 VO 64 IS 1 A1 Cauré Portugal A1 Fernanda Ruiz-Larrea YR 2013 UL http://www.ajevonline.org/content/64/1/139.abstract AB Brettanomyces/Dekkera is considered as one of the main causes of microbial spoilage and sensory deviations of red wines. This work compares the sensitivity and effectiveness of conventional microbiological culture and real-time polymerase chain reaction (Q-PCR) methods for Brettanomyces/Dekkera detection and quantification and demonstrates a positive correlation between both methods. Moreover, an improved DNA extraction protocol enabled quantification of Brettanomyces/Dekkera cells by Q-PCR down to 20 cells/mL in turbid wines in a total of 324 red wine samples. The conventional culture analysis is time-consuming but has lower cost than Q-PCR, and it is simple and efficient in quantifying viable Brettanomyces/Dekkera cells.