RT Journal Article
SR Electronic
T1 Electrophoretic Karyotypes of Wine Yeasts
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 358
OP 363
DO 10.5344/ajev.1991.42.4.358
VO 42
IS 4
A1 Nami Yamamoto
A1 Nobuki Yamamoto
A1 Hidehito Amemiya
A1 Yoichi Yokomori
A1 Kenichi Shimizu
A1 Akira Totsuka
YR 1991
UL http://www.ajevonline.org/content/42/4/358.abstract
AB Chromosomal DNAs from 77 wine yeast strains were examined by pulsed field gel electrophoresis (PFGE). Judging from the numbers and sizes of the chromosomes, the PFGE patterns of all of the wine yeasts tested were similar to that of a haploid strain, Saccharomyces cerevisiae YNN 295, whereas some significant differences were observed among the strains. Thirty-two strains showed chromosomal bands which were not found in YNN 295. All but one strain had at least one doublet band. Even when slight differences in mobility of some chromosomes were neglected, 51 different karyotypes were observed. Differences in karyotypes were also observed among the strains under the same designation. These facts indicated that PFGE would be useful and reliable for differentiation of wine yeast strains.