RT Journal Article
SR Electronic
T1 Use of F(ab')<sub>2</sub> Antibody Fragment in ELISA for Detection of Grapevine Viruses
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 38
OP 40
DO 10.5344/ajev.1992.43.1.38
VO 43
IS 1
A1 Adib Rowhani
YR 1992
UL http://www.ajevonline.org/content/43/1/38.abstract
AB A modification of the enzyme-linked immunosorbent assay using the F(ab')2 antibody fragment [F(ab')2 ELISA] was developed for the detection of grapevine fanleaf (GFLV) and grapevine leafroll (GLRV-type III) viruses. The protocol was sensitive and rapid. Sensitivity of the test was further enhanced by pre-mixing the detecting antibody (probe) and the enzyme-conjugated protein A (anti-probe). Due to the acidic nature of grapevine tissues, different extraction buffer compositions were also evaluated.