PT  - JOURNAL ARTICLE
AU  - H. Priévost
AU  - J. F. Cavin
AU  - M. Lamoureux
AU  - C. Diviès
TI  - Plasmid and Chromosome Characterization of <em>Leuconostoc oenos</em> Strains
AID  - 10.5344/ajev.1995.46.1.43
DP  - 1995 Jan 01
TA  - American Journal of Enology and Viticulture
PG  - 43--48
VI  - 46
IP  - 1
4099  - http://www.ajevonline.org/content/46/1/43.short
4100  - http://www.ajevonline.org/content/46/1/43.full
SO  - Am J Enol Vitic.1995 Jan 01; 46
AB  - Fifteen strains of Leuconostoc oenos were examined for plasmid content. Six were found to contain single cryptic plasmids designated pUBL01 to pUBL06 and ranging in size from 3.9 to 26 kb. Through size analysis, restriction endonuclease mapping, and DNA-DNA hybridization, the group of six plasmids comprised three plasmids which were not homologous. Pulsed field electrophoresis and restriction fragment length polymorphism mapping were used to differentiate strains harboring plasmid DNA. The genome size ranged from 1770 to 1975 kb. EcoRI and Hind III RFLP were suitable to differentiate the strains in the region of 9- to 23-kb fragments. Two strains with identical RFLP contained different plasmids useful for their recognition.