TY - JOUR T1 - Measurement of 3-Alkyl-2-Methoxypyrazine by Headspace Solid-Phase Microextraction in Spiked Model Wines JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 285 LP - 288 DO - 10.5344/ajev.2002.53.4.285 VL - 53 IS - 4 AU - Peter J. Hartmann AU - Harold M. McNair AU - Bruce W. Zoecklein Y1 - 2002/01/01 UR - http://www.ajevonline.org/content/53/4/285.abstract N2 - The effect of wine matrix ingredients and conditions on the headspace sampling of 3-alkyl-2-methoxypyrazines was investigated with solid-phase microextraction (SPME) and capillary gas chromatography, using a nitrogen phosphorus detector. Changes in the recovery of 3-ethyl-, isopropyl-, sec-butyl-, and isobutyl-2-methoxypyrazines from the static headspace of synthetic wine matrices spiked with 5 µg/L of each analyte were investigated and reported as a function of SPME fiber type, extraction time, and temperature. The influence of pH, ethanol, phenolics, and oak was studied. Divinylbenzene/carboxen/polydimethylsiloxane (PDMS) SPME fibers at an extraction temperature of 50°C for 30 minutes with 30% (w/v) added sodium chloride resulted in the highest analyte recoveries. Although PDMS (100 µm) SPME fibers at an extraction temperature of 35°C for 30 minutes with 30% (w/v) added sodium chloride resulted in lower analyte recoveries, the fiber remained functional for 50 to 75 analyses after other coatings deteriorated. Changing the sample ethanol concentration from 0 to 20% (v/v) resulted in an exponential decrease in the recovered analytes. Below pH 2, there was extensive loss of the analytes in the headspace. No measurable impact on alkyl-methoxypyrazine headspace concentrations was observed with exposures to selected phenolics and to oak. ER -