TY - JOUR T1 - High Performance Liquid Chromatographic Analysis of Anthocyanins in the Red Seedless Table Grape Reliance JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 339 LP - 345 DO - 10.5344/ajev.1995.46.3.339 VL - 46 IS - 3 AU - Yu Gao AU - Garth A. Cahoon Y1 - 1995/01/01 UR - http://www.ajevonline.org/content/46/3/339.abstract N2 - The anthocyanin profile of Vitis hybrid cv. Reliance, a red seedless table grape, was characterized by high performance liquid chromatography (HPLC), paper chromatography (PC), thin layer chromatography (TLC), and spectral measurements. Purified anthocyanins were obtained from Reliance grapes by descending preparative PC using Whatman 3MM paper. Reliance anthocyanins were identified by: (1) a systematic comparison with authentic anthocyanin and anthocyanidin standards by C18 reverse-phase HPLC; (2) determination of sugar residues using TLC after complete acid hydrolysis; and (3) spectral measurements. The anthocyanin profile of Reliance was shown to have seven anthocyanin peaks on a C18 reverse-phase column. The peaks 2, 3, and 5 were identified as delphinidin-3-glucoside (32%), cyanidin-3-glucoside (56%), and peonidin-3-glucoside. Peaks 1, 4, 6, and 7 were tentatively identified as cyanidin-3,5-diglucoside, petunidin-3-glucoside, malvidin-3-glucoside, and an acylated cyanidin derivative, respectively. ER -