RT Journal Article
SR Electronic
T1 Genetic Analysis of Agrobacterium tumefaciens and A. vitis Using Randomly Amplified Polymorphic DNA
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 145
OP 151
DO 10.5344/ajev.1996.47.2.145
VO 47
IS 2
A1 N. A. Irelan
A1 C. P. Meredith
YR 1996
UL http://www.ajevonline.org/content/47/2/145.abstract
AB The extent of genetic heterogeneity within and between three Agrobacterium biovars: A. tumefaciens biovars I and II, and A. vitis (biovar III) was surveyed using randomly amplified polymorphic DNA (RAPD) markers. A high level of both inter- and intraspecific genetic diversity was detected in the 20 Agrobacterium strains tested. No monomorphic bands were detected and only a small number of intraspecific monomorphic bands were identified. Biovar II strains exhibited the highest proportion of shared bands (41%, vs. 38% and 12% for biovar I and A. vitis, respectively), but only one of these shared markers was intraspecifically monomorphic. In contrast, biovar I strains had a larger proportion of shared markers that were intraspecifically monomorphic. The greatest genetic diversity was detected among the A. vitis genotypes. Although genetic variation detected between strains was high, phenetic analysis revealed that genetic similarity was highly correlated with biovar designation. Our results suggest that RAPD markers may have utility in Agrobacterium (biovar) identification and classification.