TY - JOUR T1 - Resveratrol and Stilbene Synthase mRNA Production in Grapevine Leaves Treated with Biotic and Abiotic Phytoalexin Elicitors JF - American Journal of Enology and Viticulture JO - Am J Enol Vitic. SP - 60 LP - 64 DO - 10.5344/ajev.2004.55.1.60 VL - 55 IS - 1 AU - Bruno Borie AU - Philippe Jeandet AU - Aline Parize AU - Roger Bessis AU - Marielle Adrian Y1 - 2004/01/01 UR - http://www.ajevonline.org/content/55/1/60.abstract N2 - Vitis rupestris and Vitis vinifera cvs. Pinot noir and Chardonnay plants grown in vitro were treated with defense elicitors in order to study the expression of stilbene synthase (StSy) genes. Changes in StSy mRNA production were monitored 0 to 24 hr after treatment with UV light. A constant level of expression was observed for V. rupestris (a species tolerant to gray mold), whereas there were two peaks in expression for the V. vinifera varieties Pinot noir and Chardonnay (both susceptible to gray mold). Similar profiles were observed for resveratrol accumulation, showing that stilbene synthesis is correlated to StSy mRNA production. Three elicitors of grapevine defense mechanisms—UV irradiation, aluminum chloride, and Botrytis cinerea—did not induce the same StSy mRNA production in V. rupestris. UV irradiation induced a high, constant level of StSy mRNA production correlated to resveratrol accumulation, while the other two elicitors induced irregular responses.Acknowledgments: The authors thank P. Coutos-Thevenot (Université de Poitiers) and X. Daire, G. Aubert, and E. Boudon (INRA Dijon) for valuable discussions and help. ER -