RT Journal Article SR Electronic T1 Evaluating Resistance to Grape Phylloxera in Vitis Species with an in vitro Dual Culture Assay JF American Journal of Enology and Viticulture JO Am. J. Enol. Vitic. FD American Society for Enology and Viticulture SP 17 OP 22 VO 49 IS 1 A1 Grzegorczyk, Wladyslawa A1 Walker, M. Andrew YR 1998 UL http://www.ajevonline.org/content/49/1/17.abstract AB Forty-one accessions of 12 Vitis L. and Muscadinia Small species were evaluated for resistance to grape phylloxera (Daktulosphaira vitifoliae Fitch) using an in vitro dual culture system. The performance of the species tested in this study was consistent with previously published studies with whole plants and helps confirm the utility of in vitro dual culture for the study of grape/phylloxera interactions. This in vitro system provides rapid results (8 wk) and the ability to observe the phylloxera/grape interaction without interference from other factors. This system also provides an evaluation that overemphasizes susceptibility, thus providing more confidence in the resistance responses of a given species or accession. Among the unusual responses were the susceptibility of V. riparia Michx. DVIT 1411; susceptibility within V. berlandieri Planch.; relatively wide ranging responses in V. rupestris Scheele; and the lack of feeding on the roots of V. californica Benth., in contrast to the severe foliar feeding damage that occurred on this species. Vitis californica #11 and V. girdiana Munson DVIT 1379 were unusual because phylloxera on them had the shortest generation times. Such accessions might be used to examine how grape hosts influence phylloxera behavior. Very strong resistance was found within V. aestivalis Michx. DVIT 7109 and 7110; V. berlandieri c9031; V. cinerea Engelm; V. riparia (excluding DVIT 1411); V. rupestris DVIT 1418 and 1419; and M. rotundifolia Small. These species and accessions seem to possess enough resistance to enable their use in breeding with minimal concern about phylloxera susceptibility.