RT Journal Article
SR Electronic
T1 Identification of Lactic Acid Bacteria from Vinegar Flies Based on Phenotypic and Genotypic Characteristics
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 519
OP 525
DO 10.5344/ajev.2006.57.4.519
VO 57
IS 4
A1 Willem H. Groenewald
A1 Carol A. Van Reenen
A1 Svetoslav D. Todorov
A1 Maret Du Toit
A1 R. Corli Witthuhn
A1 Wilhelm H. Holzapfel
A1 Leon M.T. Dicks
YR 2006
UL http://www.ajevonline.org/content/57/4/519.abstract
AB Thirty lactic acid bacteria were isolated from the intestinal tract of Drosophila simulans Stuvervant collected from a winery in the Stellenbosch region, South Africa. The isolates were grouped according to morphological, biochemical, and physiological characteristics. Strains selected from each group were identified to species level by PCR with species-specific primers, PCR-based DGGE, and 16S rDNA sequencing. The majority of isolates belonged to the species Lactobacillus plantarum, but Lactobacillus paracasei, Lactobacillus sanfranciscensis, Leuconostoc mesenteroides subsp. mesenteroides, Lactococcus lactis subsp. lactis, Enterococcus faecalis and Pediococcus pentosaceus were also identified. The genotypic relatedness between strains of L. plantarum was determined by RAPD-PCR.