RT Journal Article
SR Electronic
T1 A Rapid and Simple Method for Simultaneous Determination of Glycerol, Fructose, and Glucose in Wine
JF American Journal of Enology and Viticulture
JO Am J Enol Vitic.
FD American Society for Enology and Viticulture
SP 279
OP 282
DO 10.5344/ajev.2007.58.2.279
VO 58
IS 2
A1 Enzo Moro
A1 Roberto Majocchi
A1 Cinzia Ballabio
A1 Silvia Molfino
A1 Patrizia Restani
YR 2007
UL http://www.ajevonline.org/content/58/2/279.abstract
AB A simple method has been developed for assaying glycerol, glucose, and fructose in wine by high-performance liquid chromatography using a modified polar-bonded NH2-silica phase column and a refractive index detector. Glycerol, fructose, and glucose concentrations ranged from 3.30 to 7.67 mg/mL, from undetectable to 9.12 mg/mL, and from undetectable to 3.60 mg/mL, respectively, for high- and standard-quality red wines. For high- and standard-quality white wines, these concentrations ranged from 1.65 to 5.87 mg/mL, 0.18 to 7.07 mg/mL, and undetectable to 3.21 mg/mL, respectively. Fructose and glucose concentrations were much higher in sweet and sparkling wines (18.5 to 127 mg/mL). The method described measures glycerol and the two sugars simultaneously, allowing easy, specific, and rapid control of wine quality.