PT - JOURNAL ARTICLE AU - Viana, Fernando AU - Taillandier, Patricia AU - Vallés, Salvador AU - Strehaiano, Pierre AU - Manzanares, Paloma TI - 2-Phenylethyl Acetate Formation by Immobilized Cells of <em>Hanseniaspora vineae</em> in Sequential Mixed Fermentations AID - 10.5344/ajev.2010.10078 DP - 2011 Mar 01 TA - American Journal of Enology and Viticulture PG - 122--126 VI - 62 IP - 1 4099 - http://www.ajevonline.org/content/62/1/122.short 4100 - http://www.ajevonline.org/content/62/1/122.full SO - Am. J. Enol. Vitic.2011 Mar 01; 62 AB - Hanseniaspora vineae 1471, characterized as a strong producer of 2-phenylethyl acetate in wine, has been immobilized on calcium alginate beads. Fermentation trials of free and immobilized cells showed that immobilization did not affect the levels of 2-phenylethyl acetate. The removal of H. vineae immobilized cells from the fermenting must at different time points followed by the inoculation of free cells of a commercial strain of Saccharomyces cerevisiae can be used as a tool to modulate the concentration of 2-phenylethyl acetate in wine. Analysis of enological characteristics and volatile compounds showed the feasibility of the use of the mixed sequential cultures to produce wines with different chemical characteristics.