@article {P{\'e}rez-Gilabert135, author = {Manuela P{\'e}rez-Gilabert and Edgar T{\'e}llez and Francisco Garc{\'\i}a-Carmona}, title = {Extraction and Partial Purification of β-Galactosidase from Grape Berry Skin (Vitis vinifera L.) with Triton X-114}, volume = {61}, number = {1}, pages = {135--139}, year = {2010}, doi = {10.5344/ajev.2010.61.1.135}, publisher = {American Journal of Enology and Viticulture}, abstract = {Monastrell grape skins show a high percentage of galactose, likely due to low β-galactosidase activity. Little is known about the characteristics of this enzyme. A simple method to extract and partially purify β-galactosidase from grape berry skin is presented. The kinetic properties of this enzyme are comparable to those of β-galactosidase from other fruits. In this method Triton X-114 (a non-ionic detergent) has a double effect: solubilizing skin components to liberate the enzyme and removing most phenols and hydrophobic proteins, giving rise to a 56-fold increase in specific activity. The method proposed may be useful to easily extract and partially purify this enzyme during grape berry ripening.}, issn = {0002-9254}, URL = {https://www.ajevonline.org/content/61/1/135}, eprint = {https://www.ajevonline.org/content/61/1/135.full.pdf}, journal = {American Journal of Enology and Viticulture} }