RT Journal Article SR Electronic T1 Genetic Origin of the Grapevine Cultivar Tempranillo JF American Journal of Enology and Viticulture JO Am. J. Enol. Vitic. FD American Society for Enology and Viticulture SP 549 OP 553 DO 10.5344/ajev.2012.12012 VO 63 IS 4 A1 Ibáñez, Javier A1 Muñoz-Organero, Gregorio A1 Zinelabidine, L. Hasna A1 de Andrés, M. Teresa A1 Cabello, Félix A1 Martínez-Zapater, José Miguel YR 2012 UL http://www.ajevonline.org/content/63/4/549.abstract AB Tempranillo is the most important Spanish grapevine cultivar used for red wine production and it is currently planted in many other wine regions worldwide. Genotyping analysis of single nucleotide polymorphism (SNP) and microsatellite markers in grapevine germplasm collections has identified Albillo Mayor and Benedicto as the parents of Tempranillo. The statistical support for this conclusion is very high, with a LOD value of 138 for the joint estimation. Albillo Mayor is a well-known variety still cultivated in the central Iberian Peninsula. In contrast, Benedicto is rarely cultivated today and lacks historical references in the main Spanish viticulture literature. A search for putative offspring varieties derived from Tempranillo in a database containing most of the Spanish varieties identified only one variety, Coloraíllo, as a putative progeny of Tempranillo and Moravia Dulce (prime name Marufo). Analyses of chloroplast microsatellite markers identified Tempranillo as the male parent. In addition, one putative parent-offspring relationship was detected for each of the Tempranillo parents: Albillo Mayor is related to Castellana Blanca and Benedicto to Benedicto Falso de Aragón.