PT - JOURNAL ARTICLE AU - Jacopo Bigliazzi AU - Monica Scali AU - Elisa Paolucci AU - Mauro Cresti AU - Rita Vignani TI - DNA Extracted with Optimized Protocols Can Be Genotyped to Reconstruct the Varietal Composition of Monovarietal Wines AID - 10.5344/ajev.2012.12014 DP - 2012 Dec 01 TA - American Journal of Enology and Viticulture PG - 568--573 VI - 63 IP - 4 4099 - http://www.ajevonline.org/content/63/4/568.short 4100 - http://www.ajevonline.org/content/63/4/568.full SO - Am. J. Enol. Vitic.2012 Dec 01; 63 AB - A new DNA extraction protocol has been established to reconstruct the genetic identity of grapevine cultivars used for the production of experimental and commercial monovarietal wines. Seven wines originating from Merlot, Pinot noir, Zinfandel, Riesling, Sauvignon blanc, Sangiovese, and Alicante were identified by simple sequence repeat (SSR) analysis. The average quantity of Vitis vinifera DNA that can be extracted from wine, estimated by RT-PCR, was between 2,199 and 87,995 gc/mL. This is the first study that reports source vine identity reconstructed with a significant statistical correspondence (PI ≤ 10−6) in both experimental and commercial wines.