RT Journal Article
SR Electronic
T1 DNA Extracted with Optimized Protocols Can Be Genotyped to Reconstruct the Varietal Composition of Monovarietal Wines
JF American Journal of Enology and Viticulture
JO Am. J. Enol. Vitic.
FD American Society for Enology and Viticulture
SP 568
OP 573
DO 10.5344/ajev.2012.12014
VO 63
IS 4
A1 Jacopo Bigliazzi
A1 Monica Scali
A1 Elisa Paolucci
A1 Mauro Cresti
A1 Rita Vignani
YR 2012
UL http://www.ajevonline.org/content/63/4/568.abstract
AB A new DNA extraction protocol has been established to reconstruct the genetic identity of grapevine cultivars used for the production of experimental and commercial monovarietal wines. Seven wines originating from Merlot, Pinot noir, Zinfandel, Riesling, Sauvignon blanc, Sangiovese, and Alicante were identified by simple sequence repeat (SSR) analysis. The average quantity of Vitis vinifera DNA that can be extracted from wine, estimated by RT-PCR, was between 2,199 and 87,995 gc/mL. This is the first study that reports source vine identity reconstructed with a significant statistical correspondence (PI ≤ 10−6) in both experimental and commercial wines.