TY - JOUR T1 - Selection for <em>Run1-Ren1</em> Dihybrid Grapevines Using Microsatellite Markers JF - American Journal of Enology and Viticulture JO - Am. J. Enol. Vitic. DO - 10.5344/ajev.2012.12060 SP - ajev.2012.12060 AU - Chen Li AU - Alexandra Erwin AU - Dániel Pap AU - Courtney Coleman AU - Alyssa D. Higgins AU - Erzsébet Kiss AU - Pál Kozma AU - Sarolta Hoffmann AU - David W. Ramming AU - László G. Kovács Y1 - 2012/10/22 UR - http://www.ajevonline.org/content/early/2012/10/19/ajev.2012.12060.abstract N2 - A grapevine hybrid progeny was generated to track the inheritance of the Ren1 and the Run1 powdery mildew resistance alleles and the segregation of the powdery mildew resistance phenotype. Genotypic analysis was carried out using flanking microsatellite markers; phenotypic evaluations were done under in vitro and greenhouse conditions. Pairing the phenotypic and genotypic data demonstratedthat Ren1 and Run1 acted as single dominant loci and assorted independentlywithout considerable distortion of segregation. Chromosomal recombination events were detected in the Ren1 but not in the Run1 region, corroborating earlier observations that crossover between homologous chromosomes was suppressed around the Run1 locus. Taken together, the results confirmedthat microsatellite marker-assisted selection is a reliable and expeditious method to combine multiple alleles that confer resistance to a pathogen. ER -