RT Journal Article SR Electronic T1 Selection for Run1-Ren1 Dihybrid Grapevines Using Microsatellite Markers JF American Journal of Enology and Viticulture JO Am. J. Enol. Vitic. FD American Society for Enology and Viticulture SP ajev.2012.12060 DO 10.5344/ajev.2012.12060 A1 Chen Li A1 Alexandra Erwin A1 Dániel Pap A1 Courtney Coleman A1 Alyssa D. Higgins A1 Erzsébet Kiss A1 Pál Kozma A1 Sarolta Hoffmann A1 David W. Ramming A1 László G. Kovács YR 2012 UL http://www.ajevonline.org/content/early/2012/10/19/ajev.2012.12060.abstract AB A grapevine hybrid progeny was generated to track the inheritance of the Ren1 and the Run1 powdery mildew resistance alleles and the segregation of the powdery mildew resistance phenotype. Genotypic analysis was carried out using flanking microsatellite markers; phenotypic evaluations were done under in vitro and greenhouse conditions. Pairing the phenotypic and genotypic data demonstratedthat Ren1 and Run1 acted as single dominant loci and assorted independentlywithout considerable distortion of segregation. Chromosomal recombination events were detected in the Ren1 but not in the Run1 region, corroborating earlier observations that crossover between homologous chromosomes was suppressed around the Run1 locus. Taken together, the results confirmedthat microsatellite marker-assisted selection is a reliable and expeditious method to combine multiple alleles that confer resistance to a pathogen.