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A microsatellite marker based framework linkage map of Vitis vinifera L.

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Abstract

We have constructed a framework linkage map based on microsatellite markers for Vitis vinifera L., the European wine grape. The mapping population consisted of 153 progeny plants from a cross of Vitis vinifera cvs. Riesling × Cabernet Sauvignon. One hundred fifty-two microsatellite markers and one polymorphic EST marker have been mapped to 20 linkage groups (2n=38). The map covers 1,728 cM with an average distance between markers of 11.0 cM. Estimates of genome size, expected genome coverage, and observed genome coverage were determined with 135–140 markers. Genome length estimates differed between paternal and maternal data sets. Observed approximate genome coverage was 65% versus an expected coverage of 90%. Meiotic recombination rates were not significantly different between maternal and paternal parents. This map has been adopted as a reference map for the International Grape Genome Program.

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Acknowledgements

This research was supported by the American Vineyard Foundation and the United States Department of Agriculture Viticulture Consortium. We greatly appreciate the work of Dr. Peter Isaac at AgroGene and the other members of the Vitis Microsatellite Consortium in the development of new grape microsatellite markers. Thanks to Anne-Francoise Adam-Blondon, Patrice This, Stella Grando and Eva Zyprian for sharing unpublished maps.

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Authors and Affiliations

  1. Department of Viticulture and Enology, University of California, One Shields Avenue, Davis, CA 95616, USA

    S. Riaz, G. S. Dangl & C. P. Meredith

  2. IACR-Long Ashton, Long Ashton Research Station, Long Ashton, Bristol, BS18 9AF, UK

    K. J. Edwards

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  1. S. Riaz
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  2. G. S. Dangl
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  3. K. J. Edwards
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  4. C. P. Meredith
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Correspondence to C. P. Meredith.

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Communicated by C. Möllers

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Riaz, S., Dangl, G.S., Edwards, K.J. et al. A microsatellite marker based framework linkage map of Vitis vinifera L.. Theor Appl Genet 108, 864–872 (2004). https://doi.org/10.1007/s00122-003-1488-5

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  • DOI: https://doi.org/10.1007/s00122-003-1488-5

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