Abstract
Symptomless grape plants (Vitis vinifera) cultivated in Jind, Punjab, have been found to carry a Grapevine red blotch virus (GRBV). Evaluation of full length DNA sequence (3204 bp) of the virus (KU522121) has revealed similarity with mastrevirus, begomovirus, and other Grapevine red blotch viruses reported in the US and Canada. Similar to naturally growing plants, agroinfiltrated model plants with infectious clone of GRBV do not show any visible disease warning sign. To the best of our knowledge, this is the first report of a symptomless host Vitis vinifera from Indian vineyards harbouring a Grapevine geminivirus.
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Acknowledgements
The authors are thankful to Science and Engineering Research Board – Department of Science and Technology, New Delhi, India for the financial assistance (File Nos. YSS/2015/000265 and EMR/2016/000579).
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Supplementary material 1.
Vitis vinifera symptomless sample (A, B, C) collected from the grape plantation fields in Punjab. Neither the Chenopodium album (D) nor the Lycopersicon esculentum (E) plants developed any symptoms when induced with infectious construct of isolate RK-1. Even the Green House raised Grapevine plants were devoid of any observable symptoms (F). (PNG 1098 kb)
Supplementary material 2.
Dot Blot hybridization of DNA samples extracted from the infectious construct inoculated (1 to 5) and non inoculated/healthy model plants (Abbreviated as Ca = Chenopodium album, Le = Lycopersicon esculentum and Vv = Vitis vinifera) (6 to 10). (PNG 235 kb)
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Marwal, A., Kumar, R., Paul Khurana, S.M. et al. Complete nucleotide sequence of a new geminivirus isolated from Vitis vinifera in India: a symptomless host of Grapevine red blotch virus. VirusDis. 30, 106–111 (2019). https://doi.org/10.1007/s13337-018-0477-x
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DOI: https://doi.org/10.1007/s13337-018-0477-x