Micro method for the identification of proanthocyanidin using thiolysis monitored by high-performance liquid chromatography

doi:10.1016/S0021-9673(01)88830-0

Journal of Chromatography A

Volume 540, 1991, Pages 401-405

https://doi.org/10.1016/S0021-9673(01)88830-0 Get rights and content

Abstract

The classical method of procyanidin thiolysis was modified so as to render it suitable for the identification of small amount (<0.1 mg) of compound. High-performance liquid chromatographic monitoring of the degradation kinetics gives acces to the intermediate products. The use of sulphur dioxide instead of acetic acid as the hydrolysing agent allows a faster reaction, thus minimizing structural modifications, and prevents alterations due to procyanidin oxidation.

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¹: Present address: Instituto de Fermentaciones Industriales, Juan de la Cierva 3, 28006 - Madrid, Spain.

²: On leave from the Universidade Técnica de Lisboa, Instituto Superior de Agronomia, Laboratorio “Ferreira Lapa”, 1399 Lisboa codex, Portugal.

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Short CommunicationMicro method for the identification of proanthocyanidin using thiolysis monitored by high-performance liquid chromatography

Abstract

Biomed. Environ. Mass Spectrom.

Anal. Chem.

J. Chem. Soc., Perkin Trans. 1

Phytochemistry

J. Chem. Soc., Perkin Trans. 1

J. Chem. Soc., Perkin Trans. 1

J. Wood Chem. Technol.

Short Communication
Micro method for the identification of proanthocyanidin using thiolysis monitored by high-performance liquid chromatography