Original ArticlesPurification and Characterization of Two β-d-Glucosidases from an Aspergillus Niger Enzyme Preparation: Affinity and Specificity Toward Glucosylated Compounds Characteristic of the Processing of Fruits
Introduction
Commercial enzyme preparations are complex mixtures of pectinases, cellulases, hemicellulases, and glycosidases.1, 2, 3 They are used by the fruit- and vegetable-processing industry to improve the technological properties of juices (Brix level, juice yield, flavor and color extraction, filterability, clarification, and juice viscosity).1, 3, 4, 5 These enzymes can also degrade fruit cell walls to increase juice yields and improve color and flavor extraction.6, 7, 8
β-d-glucosidases (EC 3.2.1.21) present in commercial enzyme preparations participate in cellulolysis through the hydrolysis of cellobiose and contribute to aroma enhancing (particularly in wine) by hydrolyzing terpenyl-β-d-glucosides into terpenols.9, 10, 11 In red fruit juices and wines, they can induce a loss of color.5, 12 Anthocyanins, responsible for the color of red fruits,13, 14 are formed by the addition of a mono- or disaccharide to anthocyanidins. The main sugar residue of anthocyanins is glucose. It is present in fruits such as blueberry, cranberry, blackcurrant, plum, and grape.15, 16 The decolorization is due to the breaking of the linkage between the sugar residue and the anthocyanidin which degrades spontaneously into colorless compounds.17, 18 The discolorating effect of fungal preparations from Aspergillus niger on raspberry,[19] strawberry,[20] blackberry,[21] cranberry,[5] and grape21, 22 anthocyanins has been reported. In grapes juices and wines, since the main anthocyanins are anthocyanidin-β-d-glucosides the decolorization is due to β-d-glucosidases.15, 23
Fungal β-d-glucosidases have been isolated from Humicola grisea,[24] Fusarium oxysporum,[25] Gliocladium virens,[26] Neocallimatix frontalis,[27] Botrytis cinerea,28, 29 Talaromyces emersonii,[30] Trichoderma reesei,[31] Aspergilli aculeatus,[32] foetidus,[33] roseus,[34] terreus,[35] and niger36, 37, 38, 39, 40 All β-d-glucosidases from A. niger reported so far have molecular weights in the range 90–130 kDa, optimum pHs of about 4, optimum temperatures between 50–70°C, and an acidic isoelectric point in the pH range 3.5–4.5.
Some glycosidases active on anthocyanins have been partially purified from crude preparations of A. niger. Their activity on strawberries20, 41 and hawthorn extracts[22] has been reported. An endo-β-d-glucosidase from an A. niger preparation exhibited anthocyanase activity together with side activities on tannins and aroma precursors.[42] Most A. niger glucosidases have been studied for their activity on p-NP-β-d-glucoside and cellobiose but very few authors20, 43 paid attention to the degradation of anthocyanidin-β-d-glucosides. As far as we are aware, no study reported the effect of purified β-d-glucosidases on several substrates representing the different technological targets of β-d-glucosidases: cellobiose (cellulolysis), terpenyl-β-d-glucosides (flavor enhancing), and anthocyanidin-β-d-glucosides (decolorization) in comparison with the “reference” activity on p-nitrophenyl-β-d-glucoside.
One challenge in improving the enzyme preparations used for red-fruit processing, particularly for red wine production, is to obtain enzyme preparations containing glucosidases active on target substrates such as cellobiose and terpenyl-β-d-glucoside but with a low decolorization effect. In this paper, we described the purification and characterization of two β-d-glucosidases of A. niger which differ in their respective affinities and activities toward cellobiose, geranyl-glucoside, and anthocyanidin-β-d-glucosides.
Section snippets
Enzyme Preparation
An experimental enzyme preparation from A. niger (Gist-Brocades, Seclin, France) was used as source of β-d-glucosidases.
Substrates for Enzyme Assays
Cellobiose, p-NP-β-d-glucopyranoside (p-NP-β-d-glucoside), p-NP-α-l-glucopyranoside, p-NP-β-d-galactopyranoside, p-NP-α-l-arabinofuranoside, p-NP-α-l-rhamnopyranoside, and p-NP-β-d-cellobioside were purchased from Sigma (St. Louis, MO).
Geranyl-β-d-glucoside was a generous gift from Dr. Z. Günata (Unité de Recherches des Arômes et des Substances Naturelles-IPV-INRA,
Purification of Two β-d-Glucosidases from A. niger
The purification of the two β-d-glucosidases was followed by measuring in parallel β-d-glucosidase activity on p-NP-β-d-glucoside and the decolorization activity of an anthocyanin extract from V. vinifera var. cabernet franc. The first steps of purification (ammonium sulfate precipitation and hydrophobic interaction) were required to eliminate brown pigments from the crude preparation and induced a 50% loss of total and specific glucosidase activity. The β-d-glucosidase specific activity
Discussion
β-d-glucosidases are used in the fruit juice industry to improve the maceration or the liquefaction processes through their participation to cellulolysis and increase the aroma by hydrolyzing their glucosylated precursors; however, their presence in fungal enzyme preparations can induce a loss of color of red fruit juices when anthocyanidin-3-β-d-glucosides are hydrolyzed into unstable anthocyanidins. The aim of this study was to determine if all A. niger β-d-glucosidases are involved in these
List of Symbols
- .
p-NP-,
p-nitrophenyl-;
- .
HPAEC,
high performance anion-exchange chromatography
- .
Enzymes:
β-glucosidase (EC 3.2.1.21).
Acknowledgements
The authors wish to thank Gist-Brocades France SA (Seclin, France) for financial support. We also thank P. Sarni-Manchado, J. M. Souquet (Unité de Recherche des Polymères et Techniques Physico-Chimiques-Institut des Produits de la Vigne-INRA, Montpellier), and Z. Günata (Unité de Recherches des Arômes et des Substances Naturelles-IPV-INRA, Montpellier) for their respective assistance during the malvidin-3-glucoside purification, HPLC use, and for the gift of pure geranyl-glucoside.
References (53)
- et al.
The purification of a commercial pectinlyase from Aspergillus niger
Food Chem.
(1994) Partial characterization of a thermostable anthocyanin-β-glycosidase from Aspergillus niger
Food Chem.
(1983)- et al.
Purification and characterization of an extracellular β-glucosidase from the rumen fungus Neocallimastix frontalis EB188
Enzyme Microbiol. Technol.
(1991) - et al.
Apple pomaceA potential substrate for production of β-glucosidase by Aspergillus Foetidus
Lebens. Wiss. u. Technol.
(1994) - et al.
Characterization of cellulolytic enzyme components from Aspergillus terreus and its mutants
J. Ferment. Technol.
(1986) - et al.
Kinetic studies on strawberry anthocyanin hydrolysis by a thermostable anthocyanin-β-glycosidase from Aspergillus niger
Food Chem.
(1985) - et al.
A spectrophotometric assay for glucosidase I
Anal. Biochem.
(1994) - et al.
Enzymes in fruit and vegetable juice technology
Proc. Biochem.
(1978) - et al.
Solubilization of apple cell walls with polysaccharide-degrading enzyme
J. Appl. Biochem.
(1980) - et al.
The use of macerating enzymes in grape juice processing
Am. J. Enol. Vitic.
(1994)
Comparison of cellulolytic and pectinolytic treatment of various fruit pulps
Chem. Mikrobiol. Technol. Lebensm.
Glycosidase activity of enzyme preparations used in fruit juice processing
Food Technol.
Plum juice quality affected by enzyme treatment and fining
J. Food Sci.
Anthocyanin analysis as a measure of glycosidase activity in enzymes for juice processing
J. Food Sci.
Immobilized endo-β-glucosidase enriches flavor of wine and passion fruit juice
J. Agric. Food Chem.
Etude et exploitation par voie enzymatique des précurseurs d’arômes du raisin de nature glycosidique
Rev. Oenol. Tech. Vitivin. Oenol.
Monoterpenyl glycosides in plants and their biotechnological transformation
Acta Biotechnol.
β-glucosidase activity in juice-processing enzymes based on anthocyanin analysis
J. Food Sci.
Anthocyanins as food colorants-a review
J. Food Biochem.
Food colorantsAnthocyanins
Crit. Rev. Food Sci. Nutrit.
Anthocyanins
Small fruits
Fruit color destructionDecolorization of anthocyanins by fungal enzymes
J. Agric. Food Chem.
The kinetics of the decolorization of anthocyanins by fungal “anthocyanase”
J. Am. Chem. Soc.
Short communicationEffects of pectolytic enzyme treatments on anthocyanins in raspberry juice
Int. J. Food Sci. Technol.
Removal of excessive anthocyanin pigment by enzyme
Food Technol.
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2013, Journal of Biological ChemistryCitation Excerpt :Commercially available cellulase preparations are often supplemented with fungal β-d-glucosidases to increase the cellulolytic efficiency on pretreated biomass (3). Fungal β-d-glucosidases have been isolated from a number of fungal sources: Humicola grisea, Fusarium oxysporum, Gliocladium virens, Neocallimatix frontalis, Botrytis cinerea, Talaromyces emersonii, Trichoderma reesei, Phanerochaete crysosporium, Aspergillus aculeatus, Aspergillus foetidus, Aspergillus roseus, Aspergillus terreus, Aspergillus awamori, and Aspergillus niger, to name a few (4). The most common commercial β-glucosidase preparation (Novozymes SP 188; Novo Nordisk A/S, Bagsvaerd, Denmark) is produced by the cultivation of filamentous fungus A. niger (1).
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