Measuring the aromatic potential of Vitis vinifera L. Cv. Sauvignon blanc grapes by assaying S-cysteine conjugates, precursors of the volatile thiols responsible for their varietal aroma

C Peyrot Des Gachons; T Tominaga; D Dubourdieu

doi:10.1021/jf990979b

Measuring the aromatic potential of Vitis vinifera L. Cv. Sauvignon blanc grapes by assaying S-cysteine conjugates, precursors of the volatile thiols responsible for their varietal aroma

J Agric Food Chem. 2000 Aug;48(8):3387-91. doi: 10.1021/jf990979b.

Authors

C Peyrot Des Gachons¹, T Tominaga, D Dubourdieu

Affiliation

¹ Faculté d'OEnologie, Université Victor Segalen Bordeaux 2, Talence Cedex, France. oenogene@oenologie.u-bordeaux2.fr

PMID: 10956121
DOI: 10.1021/jf990979b

Abstract

The method presented for measuring the aromatic potential of Sauvignon blanc must is based on an assay of the S-cysteine conjugate precursors of three volatile thiols involved in the characteristic aroma of wines made from this grape variety: 4-mercapto-4-methylpentan-2-one, 4-mercapto-4-methylpentan-2-ol, and 3-mercaptohexan-1-ol. These compounds were released enzymatically from their precursors by percolating the must through an immobilized tryptophanase column (EC 4.1.99.1), catalyzing an alpha, beta-elimination reaction on the S-cysteine conjugate. The volatile thiols were analyzed by GC-MS, as were the deuterated analogues that had been released from synthesized deuterated precursors and were added as internal standards. The quantities of volatile thiols released under these conditions were proportional to the S-cysteine conjugate content of the must.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cysteine / chemistry*
Mass Spectrometry
Odorants*
Rosales / chemistry*
Sulfhydryl Compounds / chemistry*

Substances

Sulfhydryl Compounds
Cysteine