From a methanolic extract of neutralized apple fruit pulp, a minor compound was isolated by adsorption chromatography on both XAD and PVPP resins followed by rotation locular countercurrent chromatography (RLCC). Clean-up for subsequent spectroscopic studies was performed by preparative HPLC on RP-18 and RP-select B phases. Data available from UV, NMR and mass spectroscopy together with the results obtained by enzymatic and acid hydrolyses revealed the structure of the polar glycoside as vomifoliol 9-O-beta-D-glucopyranosyl-4-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyran oside.