Biocontrol as a strategy to reduce the impact of ochratoxin A and Aspergillus section Nigri in grapes

Int J Food Microbiol. 2011 Nov 15;151(1):70-7. doi: 10.1016/j.ijfoodmicro.2011.08.005. Epub 2011 Aug 17.

Abstract

The efficacy of two strains of Kluyveromyces thermotolerans in preventing the growth and ochratoxin A (OTA) accumulation of ochratoxigenic fungi both "in vitro" and "in situ" was evaluated. The data from this study showed that both yeast strains were able to control Aspergillus carbonarius and A. niger aggregate species growth and ochratoxin A accumulation. The inhibitory effects were dependent on the ochratoxigenic species, yeast strains, a(w) and temperature evaluated and their interactions. Over all conditions assayed, ochratoxin A accumulation was reduced from 3% to 100% and the growth rate from 11% to 82.5%, depending on conditions. These results are promising for future development of a bio-pesticide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus niger / growth & development*
  • Biological Control Agents*
  • DNA, Fungal / genetics
  • Food Contamination / prevention & control*
  • Kluyveromyces / genetics
  • Kluyveromyces / isolation & purification
  • Kluyveromyces / physiology*
  • Ochratoxins / analysis*
  • Polymorphism, Restriction Fragment Length
  • Sequence Analysis, DNA
  • Temperature
  • Vitis / microbiology*

Substances

  • Biological Control Agents
  • DNA, Fungal
  • Ochratoxins
  • ochratoxin A