Abstract
Full length cDNA clones of flavonoid 3',5'-hydroxylase, dihydroflavonol 4-reductase and flavonoid 3-glucosyltransferase were cloned from petals of Gentiana triflora. Their sequences were homologous to counterparts from other plants. Flavonoid 3',5'-hydroxylase and flavonoid 3-glucosyltransferase were enzymatically characterized by expressing cDNAs in heterologous expression systems.
MeSH terms
-
Alcohol Oxidoreductases / chemistry
-
Alcohol Oxidoreductases / genetics*
-
Alcohol Oxidoreductases / metabolism
-
Amino Acid Sequence
-
Anthocyanins / biosynthesis*
-
Base Sequence
-
Cytochrome P-450 Enzyme System / chemistry
-
Cytochrome P-450 Enzyme System / genetics*
-
Cytochrome P-450 Enzyme System / metabolism
-
DNA, Complementary
-
DNA, Plant
-
Gene Expression
-
Glucosyltransferases / chemistry
-
Glucosyltransferases / genetics*
-
Glucosyltransferases / metabolism
-
Mixed Function Oxygenases / chemistry
-
Mixed Function Oxygenases / genetics*
-
Mixed Function Oxygenases / metabolism
-
Molecular Sequence Data
-
Plant Proteins / genetics*
-
Plants / enzymology*
-
Recombinant Fusion Proteins / chemistry
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
Substances
-
Anthocyanins
-
DNA, Complementary
-
DNA, Plant
-
Plant Proteins
-
Recombinant Fusion Proteins
-
Cytochrome P-450 Enzyme System
-
Mixed Function Oxygenases
-
Alcohol Oxidoreductases
-
dihydroflavanol 4-reductase
-
flavonoid 3',5'-hydroxylase
-
Glucosyltransferases
-
flavonol 3-O-glucosyltransferase
Associated data
-
GENBANK/D85184
-
GENBANK/D85185
-
GENBANK/D85186