Abstract
A simple method for the visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity on electrophoretic gels was developed, using the fluorescent substrate fluorescein diacetate. The zymogram system allows a sensitive detection of esterase bands in only 5 min of incubation of both native and sodium dodecyl sulfate gels.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carboxylesterase
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Carboxylic Ester Hydrolases / metabolism*
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Electrophoresis, Polyacrylamide Gel / methods
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Fluoresceins
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Saccharomyces cerevisiae / enzymology*
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Sodium Dodecyl Sulfate
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Substrate Specificity
Substances
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Fluoresceins
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Sodium Dodecyl Sulfate
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Carboxylic Ester Hydrolases
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Carboxylesterase
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diacetylfluorescein