Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate

Electrophoresis. 2001 Apr;22(6):1021-3. doi: 10.1002/1522-2683()22:6<1021::AID-ELPS1021>3.0.CO;2-B.

Abstract

A simple method for the visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity on electrophoretic gels was developed, using the fluorescent substrate fluorescein diacetate. The zymogram system allows a sensitive detection of esterase bands in only 5 min of incubation of both native and sodium dodecyl sulfate gels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carboxylesterase
  • Carboxylic Ester Hydrolases / metabolism*
  • Electrophoresis, Polyacrylamide Gel / methods
  • Fluoresceins
  • Saccharomyces cerevisiae / enzymology*
  • Sodium Dodecyl Sulfate
  • Substrate Specificity

Substances

  • Fluoresceins
  • Sodium Dodecyl Sulfate
  • Carboxylic Ester Hydrolases
  • Carboxylesterase
  • diacetylfluorescein