Cacao beans must be subjected to fermentation before they are used in making chocolate, and their commercial value is related to a proper procedure. Saccharomyces rosei, Hansenula anomala, Pichia fermentans, Pichia membranaefaciens, and Trichosporon cutaneum were found in fermenting cacao beans. All species isolated during the investigation grew on cacao pulp, but only S. rosei, H. anomala, and P. fermentans exhibited fermenting capacity on the sugars of cacao pulp. Species of the genus Saccharomyces were identified as the agents responsible for the alcoholic phase of the cacao fermentation.