Hunting coronavirus by transmission electron microscopy–a guide to SARS‐CoV‐2‐associated ultrastructural pathology in COVID‐19 tissues
Transmission electron microscopy has become a valuable tool to investigate tissues of
COVID‐19 patients because it allows visualisation of SARS‐CoV‐2, but the ‘virus‐like particles’ …
COVID‐19 patients because it allows visualisation of SARS‐CoV‐2, but the ‘virus‐like particles’ …
Postmortem examination of COVID‐19 patients reveals diffuse alveolar damage with severe capillary congestion and variegated findings in lungs and other organs …
…, JD Haslbauer, R Nienhold, S Savic, H Hopfer… - …, 2020 - Wiley Online Library
… The mean postmortem interval from death to autopsy was 33.3 h (11–84.5 h). This study
received approval from the Ethics Committee of Northwestern and Central Switzerland (ID 2020-…
received approval from the Ethics Committee of Northwestern and Central Switzerland (ID 2020-…
[HTML][HTML] Canstatin, a novel matrix-derived inhibitor of angiogenesis and tumor growth
…, PC Colorado, DJ Panka, H Hopfer… - Journal of Biological …, 2000 - ASBMB
… to 40 μg/ml for 24 h in thymidine incorporation experiments (or 48 h for HUVEC). All groups
… were washed and grown for 4 h in low serum medium. After 4 h, the medium was replaced …
… were washed and grown for 4 h in low serum medium. After 4 h, the medium was replaced …
Anti-angiogenic cues from vascular basement membrane collagen
…, A Torre, G Kamphaus, Y Maeshima, H Hopfer… - Cancer research, 2000 - AACR
… Matrigel plugs were fixed in 4% paraformaldehyde (in PBS) for 4 h at room temperature
and then switched to PBS for 24 h. The plugs were embedded in paraffin, sectioned, and H&E …
and then switched to PBS for 24 h. The plugs were embedded in paraffin, sectioned, and H&E …
[HTML][HTML] Distinct antitumor properties of a type IV collagen domain derived from basement membrane
…, JA Grunkemeyer, MB Ericksen, H Hopfer… - Journal of Biological …, 2000 - ASBMB
… This culture was grown for ∼4 h until the cells reached an A 600 of 0.6. Protein
expression was induced by the addition of isopropyl-1-thio-β-d-galactopyranoside to a final …
expression was induced by the addition of isopropyl-1-thio-β-d-galactopyranoside to a final …
[HTML][HTML] Reducing immunosuppression preserves allograft function in presumptive and definitive polyomavirus-associated nephropathy
…, J Steiger, MJ Mihatsch, H Hopfer… - American Journal of …, 2010 - Elsevier
Early detection of polyomavirus BK (BKV) viremia and reduction of immunosuppression is
recommended for preventing polyomavirus-associated nephropathy (Py-VAN), but systematic …
recommended for preventing polyomavirus-associated nephropathy (Py-VAN), but systematic …
Clinical relevance of pretransplant donor-specific HLA antibodies detected by single-antigen flow-beads
P Amico, G Hönger, M Mayr, J Steiger, H Hopfer… - …, 2009 - journals.lww.com
Background. Defining the clinical relevance of donor-specific HLA-antibodies detected by
single-antigen flow-beads (SAFB) is important because these assays are increasingly used for …
single-antigen flow-beads (SAFB) is important because these assays are increasingly used for …
[HTML][HTML] Identification of the anti-angiogenic site within vascular basement membrane-derived tumstatin
…, M Manfredi, C Reimer, KA Holthaus, H Hopfer… - Journal of Biological …, 2001 - ASBMB
… The plates were then incubated for 46 h at which time cells were pulsed for 2 h with BrdUrd
(10 nm). The cells/DNA were then fixed to the wells, reacted with anti-BrdUrd primary and …
(10 nm). The cells/DNA were then fixed to the wells, reacted with anti-BrdUrd primary and …
CXCR3 mediates renal Th1 and Th17 immune response in murine lupus nephritis
…, A Peters, K Heiss, J Velden, H Hopfer… - The Journal of …, 2009 - journals.aai.org
… For urine collection, mice were housed in metabolic cages for 6 h one day before sacrifice.
Urinary albuminuria was determined by standard ELISA analysis (mice-albumin kit; Bethyl …
Urinary albuminuria was determined by standard ELISA analysis (mice-albumin kit; Bethyl …
Regulation of podocyte survival and endoplasmic reticulum stress by fatty acids
…, KN Campbell, CD Cohen, H Hopfer… - … of physiology-renal …, 2010 - journals.physiology.org
… mechanically and rotated for 1 h at 4C. … h, and the blots were blocked for 2 h with 5% milk
powder in TBS-Tween. Primary antibodies were applied overnight, secondary antibodies for 1 h…
powder in TBS-Tween. Primary antibodies were applied overnight, secondary antibodies for 1 h…