Transmission electron microscopy has become a valuable tool to investigate tissues of
COVID‐19 patients because it allows visualisation of SARS‐CoV‐2, but the ‘virus‐like particles’ …

… The mean postmortem interval from death to autopsy was 33.3 h (11–84.5 h). This study
received approval from the Ethics Committee of Northwestern and Central Switzerland (ID 2020-…

… to 40 μg/ml for 24 h in thymidine incorporation experiments (or 48 h for HUVEC). All groups
… were washed and grown for 4 h in low serum medium. After 4 h, the medium was replaced …

… Matrigel plugs were fixed in 4% paraformaldehyde (in PBS) for 4 h at room temperature
and then switched to PBS for 24 h. The plugs were embedded in paraffin, sectioned, and H&E …

… This culture was grown for ∼4 h until the cells reached an A 600 of 0.6. Protein
expression was induced by the addition of isopropyl-1-thio-β-d-galactopyranoside to a final …

Early detection of polyomavirus BK (BKV) viremia and reduction of immunosuppression is
recommended for preventing polyomavirus-associated nephropathy (Py-VAN), but systematic …

Background. Defining the clinical relevance of donor-specific HLA-antibodies detected by
single-antigen flow-beads (SAFB) is important because these assays are increasingly used for …

… The plates were then incubated for 46 h at which time cells were pulsed for 2 h with BrdUrd
(10 nm). The cells/DNA were then fixed to the wells, reacted with anti-BrdUrd primary and …

… For urine collection, mice were housed in metabolic cages for 6 h one day before sacrifice.
Urinary albuminuria was determined by standard ELISA analysis (mice-albumin kit; Bethyl …

… mechanically and rotated for 1 h at 4C. … h, and the blots were blocked for 2 h with 5% milk
powder in TBS-Tween. Primary antibodies were applied overnight, secondary antibodies for 1 h…